The Quality Standards And HPLC Fingerprint Study Of Lonicera Similis Hemsl From Sichuan And Chongqing

objective:Lonicera similis Hemsl from sichuan and chongqing are dry bud or with The flowers open in early.It belongs Lonicera macranthoides Hand.-Mazz. And Lonicera hypoglauca Miq.and Lonicera confuse DC. Of Caprifoliaceae plants.The uses of which includes Antibacterial anti-inflammatory, heat detoxification, cold wind scattered the effect of heat.It is mainly used for the treatment of carbuncle swollen boils, Houbi, erysipelas, fever sera dysentery, Fengreganmao, incidence of diseases such as warm.Its main effective part is Chlorogenic acid, Iso-chlorogenic acid and total flavonoids and et. Lonicera similis Hemsl are the mainstream varieties of Flos Lonicerae from sichuan.This issue will study the Pharmacognostical features,and it will identificate Lonicera similis Hemsl by TLC, and determinate its effective components, while fingerprinting studies them to enhance and improve the quality of Lonicera similis Hemsl from sichuan and chongqing. Method:For controling the quality standards of Lonicera similis Hemsl from sichuan and chongqing fully, This study using the original plant identification, trait identification, microscopic identification and chemical identification (TLC) method to make a Pharmacognosy Identification.This paper qualitative identificates chlorogenic acid in Lonicera similis Hemsl from sichuan and chongqing using TLC,and determines the total chlorogenic acid and total flavonoids content by ultraviolet spectrophotometry. While using high-performance liquid chromatography (HPLC) to carry out the content of chlorogenic acid, and using HPLC to research its fingerprint. Those could provide a scientific basis for development and utilization of Lonicera similis Hemsl from sichuan and chongqing.Results:The original plants are semi-evergreen and perennial woody plant vines. Young'branches, petiole, and peduncle length were carried out in short brown coarse hair, short hair and sparse hairs soft. Double flowers grow solitary and axillary or terminal branches into a few clusters of racemes. Herbs have the features of small wooden club-shaped flower, yellow brown surface, red brown or green, non-glandular hairs and scattered glandular hairs. Pollen microscopic characteristics with the two kinds of glandular cells, thick-walled non-glandular cells, thin-walled single-celled non-glandular hairs, pollen grains are class spherical, with three-hole groove, there are sharp edges and corners small clusters of calcium oxalate crystal. Using thin-layer chromatography identificates of the legal nature of chlorogenic acid,chlorogenic acid can be detected In the thin-layer chromatography.Using ultraviolet spectrophotometry to detect total flavonoids and total chlorogenic acid, Total flavonoids can be measured in 0.01-0.08 mg/mL with good linearity within the scope relationship (R=0.9998), recovery was 97. 71%, and RSD was 1.48%. Using HPLC to determinate chlorogenic acid,while in the range of 0.8-8μg it has good linear relationship (r=0.9998), the average recovery was 98.34%, and RSD was 0.91%.Using HPLC to carry out fingerprint study.It have been measured a total of 11 peaks,and the parameters of a total of peak accord with "traditional Chinese medicine injection fingerprinting requirements" of the relevant provisions. Conclusion:The TLC show Lonicera similis Hemsl from sichuan and chongqing contains chlorogenic acid composition; Ultraviolet spectrophotometry detecting their total flavonoids and total chlorogenic acid content. The method is simple, rapid, sensitive and with good repeatability and recovery.It can be used as the content of total flavonoids and total chlorogenic acid determination of Lonicera similis Hemsl from sichuan and chongqing. Using HPLC to determinate chlorogenic acid, This method is simple, rapid,efficient, sensitive, accurate and with good repeatability and recovery, it can be used as a quantitative analysismethod for Lonicera similis Hemsl from sichuan and chongqing. Using HPLC carried out its fingerprint study,and the method is accurate and reproducible.The established standards of fingerprints can be used as fine macranthoin of quality evaluation and an important basis for identification of species.