Association Study On Short Tandem Repeats D6S289, D6S1610 And Schizophrenia Among Han People In Jiangxi Province

Objective:Schizophrenia,SP is one kind of the most commonly and important world-wide spiritual dieseases, the incidence rate of which is about 1%, the social burden caused by which has placed as the top forth among the all other different illness. SP is a multi-genetic inherited disease, but the position for its influential genes is still unidentified. Therefore, the paper made a research about the association between Short Tandem Repeats,STR such as D6S289and D6S1610 and SP, based on case-control s association analysis and multi-functional inherited statistic software (PowerStatsV12,SPSS13.0,Matlab7.0 and Excel).Methods:Schizophrenia cases are provided by the Jiangxi Provincial Mental Hospital.They are Jiangxi Han patients with schizophrenia, a total of 306 patients, 162 patients were male, female 144, average age (36.2±6.9) years.248 persons in control group, samples from the clinical laboratory of First Affiliated Hospital of Nanchang University .They are healthy adults in Jiangxi Han, verified no significant mental and physical illness, no clear genetic relationship.Diagnostic criteria for schizophrenia refers to schizophrenia dispute standard ICD10 of the International Classification of Diseases 10th edition, with reference to our diagnostic criteria and classification of mental disorders program of the second edition revised (CCMD-II-R). ,DNA extracting uses the rapid genomic DNA extraction kit (solution type), using PCR gradient amplification of Model MG96G,using capillary electrophoresis to detect polymorphic of distribution of D6S289, D6S1610 STR loci in schizophrenia patients and control groups,analysing data by a variety of software such as PowerStatsV12, SPSS13.0, Matlab7.0 and Excel and so on, and exploring association among STR loci and schizophrenia by case - control association analysis , verifying Hardy-Weinberg equilibrium of control group with method of fit test.Results: 1. Analysis results about Hardy-Weinberg equilibrium test of two STR loci in Schizophrenia control groupOf the control group, observed values and expectated values of D6S289 and D6S1610 alleles are test by Hardy-Weinberg equilibrium testing goodness of fit, each allele P> 0.05, consistent with Hardy-Weinberg equilibrium2. 2 loci allele frequency distribution in schizophrenia patient group and control groupThere are 9 alleles detected in D6S289, and there are 8 in D6S1610.Observed group is compared and control group: detection rate of D6S289-215 in schizophrenia group 5.7%, and 11.8% in the control group(P = 0.043, RR = 0.45), the gene frequency were statistically significant (P <0.05), and RR <1, it shows D6S289-215 might be a schizophrenia protective gene.Detection rate of D6S1610-131 in schizophrenia group was 1.5%,and 5.4% in the control group (P = 0.036, RR = 0.26), the gene frequency were statistically significant ( P <0.05), and RR <1, it shows that the gene is probably a schizophrenia protective gene; detection rate of D6S1610-137 was 4.4% in schizophrenia group,and 0.7% in the control group (P = 0.037 , RR = 6.55), the gene frequency were statistically significant (P <0.05), and RR> 1, it shows that the gene may be a risk gene of schizophrenia.The gene frequency of D6S1610-131, D6S1610-137 and D6S289-215 were statistically significant (P <0.05), other allele frequency between the two groups were not statistically significant (P> 0.05).Conclusions:The result about association study on D6S289 and schizophrenia shows that D6S289-215 may be a protective genes for schizophrenia.The result about association study on D6S1610 and schizophrenia shows that D6S1610- 131 may be a protective gene for schizophrenia, and D6S1610-137 may be a risk schizophrenia susceptibility gene.