The Changes Of Rat Myocardial EGR-1, CYR61 And CTGF After Exhaustive Exercise At Different Time Courses

Objective:In this study,analyzed and summarized myocardial EGR-1,CYR61 and CTGF protein in the characteristics of phase change and the rules after exhaustive through the exhaustive swimming to simulate the occurrence of exercise-induced myocardial micro-injury, in order to explore the protection mechanism and the mechanism of ventricular remodeling about exercise-induced myocardial micro-injury.Methods:This study selected methods of immunofluorescence histochemistry and image analysis technology to study the phase changes of myocardial EGR-1,CYR61 and CTGF after exhaustive, based with exhaustive swimming exercise of rat myocardial movement to establish experimental animal models of micro-damage.Result:(1)Myocardial EGR-1 protein content of each part of the heart were significantly increase(P<0.05) after both single bout and repeatedly exhausted exercise group,while it has significantly decrease(P<0.05) after 24hours single bout,and it has significantly lower(P<0.05) than the control group and the single bout with the same time-phasein 6,12,24hours consecutive daily exhausted exercise group.(2) Myocardial CYR61 protein content of each part of the heart were significantly increase(P<0.05) after both single bout and repeatedly exhausted exercise group, while it has significantly decrease(P<0.05) after 6 hours single bout, and it has significantly decrease(P<0.05) in 6 hours consecutive daily exhausted exercise group.(3) Myocardial CTGF protein content of each part of the heart were significantly increase(P<0.05) after both single bout and repeatedly exhausted exercise group, while it has significantly decrease(P<0.05) after 12,24hours in single bout. Conclusion:(1)Different exhausted exercise, various parts of myocardial EGR-1 protein content showed increase-decrease changes,EGR-1 as a key factor, can induce the cardiovascular cell apoptosis,inflammatory response,decrease the content of Ca2+,finally lead to the damage of myocardium.(2) Different exhausted exercise, various parts of myocardial CYR61 protein content showed increase-decrease changes. CYR61 can protect from serious myocardial injury. But while CYR61 protein content decreasing, it reduce the protection to myocardium, These dual features are characterized in the damage of myocardium.(3) Different exhausted exercise,various parts of myocardial CTGF protein content showed increase-decrease changes. CTGF play an important role in Fibrotic myocardium damage, finally lead to the damage of myocardium. And at the same time, the decreasing of the content of CTGF means that it s a immediate early factor.(4)Different exhausted exercise, EGR-1,CYR61,CTGF protein content showed no significant differences in right ventricular, left ventricular and interventricular septum of myocardial.