| Many studies show that intensive exercise (especially exhaustive exercise) leads to the excessive excitement of thesympathetic nerve,the decrease of gastric mucosal blood flow, meanwhile creation of free radicals, even the formation of exercise stress ulcer. Lots of researching achievements about EA have made antioxidation in many fields such as medicine, health care etc, however relevant literatures about the effects of EA on free radicals metabolism in the gastric tissure of trained rats have not been seen. Some studies showed L-Arg is the substitute of NO,it has many kinds of bioeffect such as dilating the vessel and restricting the aggregation of platelet and also protect the gastric mucosa ,while the preventive function of L-Arg to exercise stress ulcer have not been seen.The purpose of this study is to investigate effects of EA and L-Arg on growth of rats, exercise performance, ulcer index, concentrations of some brain-gut peptide and free-radical-defence systerm,and to discusse the possible mechanis, so that supply experimenu evidence for their application in the field of sports .40 male SD rats were randomly divided into: rest control group (C),training group (T),and Electroacupuncture+training group(EA+T), L-Arginine+training group (L-Arg+T), L-Arginine+Electroacupuncture+training group (L-Arg+ EA++T). Rats of C were killed in quiet, the rest groups were killed at the end of the exercise .The coefficient of the heart ,the index of the liver, spleen were tested to investigate the effect of EA on the growth of the trained rats.The activity of SOD, GSH-Px,H+, K-+ATPase , ATPase and the content of -SH, MDA were measured to reflect the status of free-radical-defense systerm. The amount of GAS, ET were tested by the method of the radio-immunity to reflect the effect of EA and L-Arg on the brain-gut petide oftrained rats.The results show: (1)Rats who treated by EA and L-Arg can significantly ran longer time till exhaustive than that werenot treated, moreover the drug administration combined with the EA is more effective, so they have the effect of antifatigue, Rats who treated by EA grow very well. (2)EA prominently restrained the decreased of the spleen ,and EA combined with the L-Arg can prominently restrained the decrease of index of the liver, spleen.This suggests that EA has different protective effects of therats'apparatus. Moveover the L-Arg combined with the EA is more effective (3) EA or EA with L-Arg can remarkablely restrain the increase of index of ulcer which caused by exhaustive exercise. This suggests that EA has a considerable prevention on the gastric mucosa, in adition the EA combined with the L-Arg is more effective.(4) EA or L-Arg can significantly increase the activity of SOD, GSH-Px, the content of-SH,and decrease the the content of MDA in the proventriculus.This shows that EA and L-Arg could act on enchancing antioxidant,clearing the free radicals,protecting gastric mucosal, preventing exercise stress ulcer,moreover the treatment by combined application of EA and L-Arg is better that by single EA or L-Arg.(5)EA can obviously restrained the decrease of the activity of Ca2+, Mg2+-ATPase, Na+, K+-ATPase, H+, K+-ATPase in the proventriculus of exhaustive exercise rats , L-Arg. can evidently restrained the decrease of the activity of Na+, K+-ATPase, H+, K+-ATPase. This results show that EA and L-Arg can protect the activity of different kind of ATPase to a different extent as well as prevent exercise stress ulcer,moreover they have synergistic effect. (6) EA can prevent the increase of the amount of GAS, ET in medulla, blood and significant restrain the increase of the amount of GAS, ET in antrum.This shows that ET can suppress gastric acid secretion and make GMBF increase by regulating the amount of GAS, ET,moreover EA regulates gastrointestinal functions by influing the content of brain-gut peptide in local tissues.(7)L-Arg can restrain the increase of the amount of ET in blood, antrum,because L-Arg doesn't pass through the blood-brain barrier. This suggests that L-Arg can in...
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