The Study About The Recovery Of Nerves Function In Rats Of Spinal Cord Injury By Step Training

Objective:To observate the effect of nerves function in rats of spin cord injury by step training,as well as determine the expression of BDNF and NT-3 in the anterior horn of gray matter. Elaborate the effect of BDNF and NT-3 in promoting the neurological functional recovery of rats with spinal cord injury by step training.Method:126 three-month-old female SD rats were randomly divided into injury control group (A group), walking training group (B group),13 rats in each group.2 with NYU spinal cord attacking implement,form 25mm dorp,attacking T9 spinal cord and making the rat bring about middle degree injury. the training group received gait training intervention after a week, the damage control group was not to intervene.3 use BBBscores and Reuter motor sensory function scores for assessment of neurological function in rats per week after modeling.4 detect BDNF and NT-3 expression in rats with spinal cord injury using the method of immunochemical and Western blotting, what was analyzed with the relationship between recovery of neurological function in rats.Results:1 The BBB motor function score Results:3 weeks later (2 week after walking training), the score of A group was significantly higher than B group at the same time-point, scores were significant differences in the third week (P<0.05), B group was (5.7±1.03) points, A group was (4.4±0.77) points, the score value with a very significant difference (P<0.01) was from the 4th week to 8th, the score values of 9th week were significant differences (P<0.05), B group was (11.0±1.06)points, A group was (9.7±0.87)points. B group self-control score had more differences from the 2th week to the 8th week.the points between 8th week and 9th week had no difference,the score of the 8th week was (11.8±0.83) points. A group of self-control score had more differences from the 3th week to the 6th week, there was no difference after 6 weeks, the score of the 6th week was (8.7±0.49) points.2 The Reuter sensory function score Results:the score of A group was significantly higher than B group at the same time-point after 4 weeks (3 weeks after step training),the differences continued until 8 weeks, the score values of the 4th,5th,6th,7th weeks had very significant differences (P<0.01). Score in the 9th week had not a difference, B group was (1.9±0.31)points, A group was (2.8±0.79)points. B group self-control score had more differences from the 3th week to the 7th week.there was no difference after 7 weeks,the score of the 7th week was (2.5±0.53) points. A group of self-control score had more differences from the 4th week to the 6th week, there was no difference after 6 weeks, the score of the 6th week was (4.3±0.61) points.3 The Western blotting results of BDNF between A group and B group had a very significant difference (P<0.01). The Western blotting results of NT-3 between A group and B group had a very significant difference (P<0.01).4 The correlation coefficient r between the BBB score and the Reuter score in training group was-0.742,tested the correlation coefficient r, P<0.01, illustrated that the BBB score had a negative correlation with the Reuter score. the recovery of motor function had relevance with sensory functional recovery.5 the correlation coefficient r between BDNF and the BBB score was 0.868, test the correlation coefficient r, P<0.01, illustrated that BDNF had a positive correlation with the BBB score, the correlation coefficient r between BDNF and the Reuter score was-0.644, test the correlation coefficient r, P<0.05, illustrated that BDNF had a negative correlation with the Reuter score. Showed that the increasing number of BDNF aroused by step training can improve neurological function in rats.6 the correlation coefficient r between NT-3 and the BBB score was 0.634, test the correlation coefficient r, P<0.05, illustrated that NT-3 had a positive correlation with the BBB score. the correlation coefficient r between NT-3 and the Reuter score was-0.656, test the correlation coefficient r, P<0.05, illustrated that NT-3 had a negative correlation with the Reuter score. Showed that the increasing number of NT-3 aroused by step training can improve neurological function in rats.Conclusions:Step training can promote neurological function in rats after spinal cord injury, the mechanism may be related to the overexpression of BDNF and NT-3 that promoted by training around the site in organizations of spinal cord injury.