The Effects Of Aerobic Exercise And Dietary Control On Cardiomyocyte Apoptosis In Type 2 Diabetic Rats

Objective:To explore the effect of aerobic exercise and dietary control on cardiomyocyte apoptosis in type 2 diabetic rats and analyze the changes in other relevant indicators.Method:8 rats were randomly selected as normal control group (Control, C)from 62 6-week-old male SD rats, fed a standard normal diet. The remaining 54 SD rats which were feed high-fat and hgh-carbohydrate diet were injected low-dose streptozotocin though abdominal cavity to establish animal models of type 2 diabetes. And then the type 2 diabetes rats were randomly divided into 4 groups: DM control group(n=9), DM+ aerobic exercise training group(DME, n=10), DM+ Diet control group(DMD, n=10), DM+ aerobic exercise training + Diet control group(DMED, n=10). The DM group's rats which were continued to feed high-fat and high-carbohydrate diet and did not exercise training; Exercise were used 60 minutes'unloaded swimming for 13 weeks, 6 times per week; The dietary control use the same amount of the standard diet in the DM group.13 weeks later, FPG, Rate of Cardiomyocyte Apoptosis, Bcl-2, Fas, Cardiomyocyte ultra-structure in every group's rats were examined.Results:â‘ After 13 weeks of the establishment of type 2 diabetes model, compared with C group, the FPG contents increased significantly in DM group(P<0.01); Through the double factor variance analysis, aerobic exercise could lower FPG levels in diabetic rats(P<0.05), and the effect of dietary control on FPG levels in diabetic rats did not significantly(P>0.05), exercise joint dietary control did not display an obvious interaction on lowering FPG levels in diabetic tats(P>0.05).â‘¡After 13 weeks of the establishment of type 2 diabetes model, compared with C group, rates of cardiomyocyte apoptosis increased significantly in DM group(P<0.01); The Bcl-2 contents decreased significantly (P<0.05) and the Fas contents increased significantly (P<0.01);â‘¢Through the double factor variance analysis, aerobic exercise or dietary control could significantly lower rates of cardiomyocyte apoptosis in diabetic rats (P<0.01), the Bcl-2 contents of cardiomyocyte increased but not significance(P>0.05), the Fas contents decreased singnificantly(P<0.01); But aerobic exercise and Dietary control could lower rates of cardiomyocyte apoptosis in diabetic rats(P<0.01), and had significantly obvious interaction to the contents of Bcl-2 and Fas of cardiomyocyte (P>0.05), which made the rate of cardiomyocyte apoptosis and contents of fas in cardiomyocyte lowered significantly in diabetic rats(P<0.01), and boosted the contents of Bcl-2 higher(P<0.01).â‘£After 13 weeks of the establishment of type 2 diabetes model, compared with C group, the construction of cardiac myfibrills is fracture derangement, and parts of were disruption and dissolution; mitochondrion was hyperplasia, some of mitochondrion had vacuoles and shrinkage in cardiomyocyte of DM group; But the morphous of cardiac myfibrills is basicly normal in cardiomyocyte of DME group, DMD group and DMED group; In these groups, the Z band arrangement is normal, no obvious disruption and dissolution; Although the size of mitochondrion was different, but the morphous and construction almost normal.Conclusion:â‘ Diabetes model in this study is successful. In the treatment of diabetes, relying on dietary control simply could ont effectively reduce the FPG and aerobic exercise training had a very significant role to control the FPG in diabetic rats, on the base of the dietary control, aerobic exercise training was more effective to reduce the fasting plasma glucose levels in diabetic rats.â‘¡The rates of cardiomyocyte apoptosis was increasing in diabetic rats, and its important mechanism would be the decrease of the Bcl-2 contents and the increase of the Fas contents.â‘¢Aerobic exercise or dietary control could significantly lower rates of cardiomyocyte apoptosis in diabetic rats; Aerobic exercise or dietary control might decrease the Fas contents in cardiomyocyte to decrease the cardiomyocyte apoptosis. At same time, aerobic exercise and dietary control had obvious interaction to decrease cardiomyocyte apoptosis in diabetic rats.â‘£When cardiomyocyte apoptosis increased in diabetic rats, the ultra-structure of cardiomyocyte were destroyed at the same time, but Aerobic exercise or dietary control could significantly decreased the distruction, and had important effects to prevent diabetic cardiomyopathy.