The Effects Of Aerobic Exercise And Dietary Control On Hemorheology In Type 2 Diabetic Rats And Its Mechanism

Objective: To investigate the effects of aerobic exercise combined with dietary control on hemorheology in type 2 diabetic rats and its mechanism.Methods:,8 rats were randomly selected rats as normal control group(Control,C)from 62 6- week-old male SD rats,fed a standard normal diet. The remaining 54 SD rats which were feed high-fat and carbohydrate diet were injected low-dose streptozotocin though abdominal cavity to establish animal models of type 2 diabetes. And then the type 2 diabetes rats were randomly divided into 4 groups:DM control group(n=9),DM+ aerobic exercise training group(DME,n=10),DM+ Diet control group(DMD,n=10),DM+ aerobic exercise training + Diet control group(DMED,n=10). The DM group's rats continued to feed high-fat and high-carbohydrate diet and did not exercise training;Exercise were used 60 minutes'unloaded swimming for 13 weeks,6 times a week;The dietary control used the same amount of the standard diet in the DM group. 13 weeks later,FPG,FINS,hemorheological indexes, plasma TXB2,PGI2 content were examined in each group of rats.Results:â‘ After 13 weeks of the establishment of type 2 diabetes model,compared with C group, the FPG contents increased significantly in DM group(P<0.01),the FINS contents decreased significantly; Through the double factor variance analysis,aerobic exercise could lower FPG levels in diabetic rats(P<0.05),FINS levels of diabetic rats further increased,but no significant interaction(P>0.05),and the effect of dietary control on FPG levels in diabetic rats did not significantly(P>0.05),But can significantly increase the FINS level of diabetic rats(P <0.05); exercise joint dietary control did not display an obvious interaction on lowering FPG and FINS level in diabetic rats(P>0.05).â‘¡After 13 weeks of the establishment of type 2 diabetes model,compared with C group,among the hemorheological indexes in the DM group,whole blood viscosity in middle shear,plasma viscosity,whole blood reduced viscosity in high shear,erythrocyte aggregation index,erythrocyte rigidity index were significantly higher,ESR and erythrocyte degeneration index decreased significantly. Through the double factor variance analysis,13 weeks of moderate-intensity swimming training could decrease the whole blood viscosity under different shear rate type 2 diabetic rats,in particular whole blood viscosity in 1:00 shear had a significant difference(P<0.05);Reduced viscosity in high-shear and low shear(P<0.01)and ESR(P = 0.05)were significantly increased; erythrocyte aggregation index decreased significantly (P <0.01),plasma viscosity,hematocrit,erythrocyte rigidity index,erythrocyte deformability index had some improvement,but there were no significant difference(P> 0.05). After the dietary control in type 2 diabetes,the erythrocyte aggregation index decreased significantly,other indicators had some improvement,but there were no significant difference. Aerobic exercise combined with diet control could further reduce the whole blood viscosity in low shear,plasma viscosity,whole blood reduced viscosity in high shear or low shear and erythrocyte aggregation index,and further increased ESR and erythrocyte deformability index in type 2 diabetic rats significantly,they had a significant interaction (P <0.05).â‘¢After 13 weeks of the establishment of type 2 diabetes model,compared with C group,in the DM group of rats plasma TXB2 concentration was significantly increased(P<0.01), PGI2 concentration decreased,but it was no significant difference(P>0.05), while TXB2/PGI2 ratio was significantly increase(P<0.05). By two-factor analysis of variance,13-week moderate-intensity swimming training on type 2 diabetes rats could lower plasma TXB2 concentration significantly(P<0.05),plasma PGI2 concentration was increased,TXB2/PGI2 ratio decreased,but there were no significant difference(P>0.05). Dietary control on type 2 diabetes could decreased plasma TXB2 concentration and TXB2/PGI2 ratio,increased PGI2 content slightly,but there were no significant difference(P>0.05). It was no significant interaction of aerobic exercise combined with dietary control on plasma TXB2,PGI2 and TXB2/PGI2(P>0.05)in type 2 diabetes.Conclusion:â‘ Diabetes model in this study is successful. In the treatment of diabetes,relying on dietary control simply could not effectively reduce the FPG. And aerobic exercise training had a very significant role to control the FPG in diabetic rats,on the base of the dietary control,aerobic exercise training was more effective to reduce the FPG levels in diabetic rats.â‘¡Blood in type 2 diabetes occurred highperviscosity,which showed concentration,coagulation,accumulation and sticky state. Aerobic exercise could improve hemorheological index in type 2 diabetic rats,lower high blood viscosity state significantly. Relying on dietary control simply could not effectively improve hemorheological index in type 2 diabetic rats. Aerobic exercise training combined with dietary control was more effective to improve hemorheological index in type 2 diabetic rats to some extent.â‘¢Plasma TXB2 significantly increased,PGI2 content decreased and TXB2/PGI2 significantly increased in diabetic rats might be one of the mechanisms of causing changes of hemorheology;Aerobic exercise,diet control,aerobic exercise combined with diet control could improve blood rheology in diabetic rats by altering plasma TXB2 and PGI2 levels.