| Purpose:an acupoint from molecular level EA on Gi, Gs protein expression of my ocardial ischemia in mice, experiments with two chronic stress stimulation, emotional f actors combined to aggravate myocardial ischemia injury, observation Effect of electroa cupuncture of Neiguan on inflammatory cytokines and myocyte Gi, Gs protein express ion. Mechanism of Chronic Stress Depression merger within the Mechanism of myocar dial ischemia myocardial ischemia treatment Neiguan Neiguan and treatment, as well a s the association between Neiguan meridians and viscera and heart, and emotional fact ors in the incidence of myocardial ischemia provide a theoretical basis and treatment of correlation among.Material and method:In the study of acupuncture Point on Myocardial Ischemi a in Mice cardiomyocytes Gi, Gs protein expression experiments, select 24 C57 BL / 6（SPF grade） and 21 mouse strains with acid-sensitive ion channel 3（SPF level） knoc kout mice were randomly divided into five groups, the normal group, model group, th e customs group, missing out group and non-acupoint group, give them more subcutan eous injection of isoproterenol way to create a model of myocardial ischemia, observ e the power to Neiguan and EA columns missing group, EA non-acupoint group ECG segment value ST and T wave worth changing; analysis Gi, Gs protein expression o bserved by light microscopy myocardial tissue morphology using Western-blot method Science changes. Off Point chronic stress in rats with myocardial ischemia inflammato ry cytokines IL-1, IL-2, IL-8 and cardiomyocytes Gi, Gs protein expression experiment s, selected 50 SD rats in the study of acupuncture were randomly divided into five gr oups, namely normal group, model group, myocardial ischemia, myocardial ischemia as sociated with chronic stress depression model group, the customs treatment group 1, th e customs treatment 2 group, give them more and subcutaneous injection of isoprotere nol forced swimming method to establish binding and after myocardial ischemia associ ated with chronic stress model of depression in the electro-acupuncture treatment grou p 1 shut off within 2 treatment groups, using Western-blot method to detect myocardi al cell Gi, Gs affect protein expression by ELISA detection of plasma IL-1, IL-2, IL-8 levels of.Results:1.C57BL/6 and ASIC3-/- mice after two kinds of modeling, in addition to the normal group, the mice in each group ST segment value and the value of T-wave elevation v arying degrees, the difference was statistically significant（P<0.05）, indicating myocard ial ischemia model was successfully established. After seven days acupuncture interven tion, the customs group, missing out group and non-acupoint group ST segment were lower, the difference in each group and this group after modeling was statistically sig nificant（P<0.05）, with the model group there are differences（P<0.05） after 7 days co mpare acupuncture intervention. Neiguan group ST segment depression more obvious,and there was significant lack of column group and non-acupoint group difference（P<0.05）. But not reduced to normal levels. Tip: Inside of power to Neiguan can be sign ificantly improved in mice, abnormal electrocardiogram ST segment elevation value an d the value of T wave acute myocardial ischemia injury, and promote the recovery of myocardial ischemic ST segment and T wave value value, missing Point and non-acu point intervention difference in Neiguan group. C57BL/6 mice off group and ASIC3-/-mice off groups, C57BL/6 mice in the group of ST segment and T wave value value decreases more pronounced.2.Detected by the light microscope morphological changes of myocardial tissue of myo cardial ischemia, Neiguan acupuncture, missing holes and have a certain effect non-acu point on myocardial ischemia in two mouse cardiomyocytes, but EA on Neiguan C57BL/6 and ASIC3-/- mice cardioprotective effect is more obvious.3.C57BL/6 and ASIC3-/- two mouse with the normal group after modeling, model grou p, the customs group, missing out group, non-acupoint group of C57BL/6 mice protein expression were decreased Gs, Gi the increased protein expression were statistically s ignificant（P<0.05）; compared with the model group, the customs group, missing out g roup Gs protein expression have risen, Gi protein expression were decreased, and stati stically significant（P<0.05）; Neiguan group and missing out relatively Gs proteins exp ression increased more significantly, Gi protein expression decreased more significantly,and statistically significant（P<0.05）. Tip: electric needle off points can significantly i ncrease C57BL/6 and ASIC3-/- two mouse Gs protein expression, reduced expression o f both proteins in mice Gi, were not able to return to normal levels, missing in the g roup did not set off effect is obvious and statistically significant（P<0.05）, non-acupoin t group had no significant effect. C57BL/6 mice off group and ASIC3-/- mice off grou ps, C57BL/6 mice in the group Gi, Gs protein expression is more obvious.4.Compared with the normal group, model group, myocardial ischemia, myocardial isc hemia associated with chronic stress depression model group, serum CK-MB, LDH tha t has increased, and was statistically significant（P<0.05）. Tip myocardial ischemia mo del was successful. With the normal group, myocardial ischemia model group and the customs treatment 1 group, myocardial ischemia associated with chronic stress and de pression group within the customs treatment slows weight gain in rats 2 groups, reduc ing sugar intake, the difference was statistically significant（P<0.05）, prompted modelin g methods can be induced in rats with chronic stress depression occurs. Compared wit h the normal group, model group myocardial ischemia and myocardial ischemia associ ated with chronic stress depression model group, serum IL-1, IL-2, IL-8 levels increas ed, increased expression of Gi, Gs protein expression decreased, and protein expressio n compared with myocardial ischemia model group, serum myocardial ischemia associa ted with chronic stress depression model group IL-1, IL-2, IL-8 levels increased more significantly, Gi; statistically significant（P<0.05） increased more significantly, Gs prot ein expression decreased more pronounced and statistically significant（P<0.05）, describ ed chronic stress depression can aggravate inflammatory factor levels, increasing Gi pr otein expression increased, adding to Gs protein expression decreased. Electro-acupunct ure of Neiguan after myocardial ischemia myocardial ischemia associated with chronic stress depression model group and with, the customs treatment group 1 and group 2within the customs treatment serum IL-1, IL-2, IL-8 decreased protein expression redu ced Gi, Gs protein expression increased, and was statistically significant（P<0.05）; Nei guan 2 treatment groups, the customs treatment group 1 IL-1, IL-2, IL-8 decreased m ore significantly, more significantly reduced expression of Gi, Gs protein expression in creased more pronounced and statistically significant（P<0.05）. Tip: In EA Neiguan can significantly improve myocardial ischemia associated with chronic stress depression i n rats serum IL-1, IL-2, IL-8 content, improve Gi, Gs protein abnormal expression, bu t within the EA Neiguan myocardial ischemia simple indicators improved compared wi th myocardial ischemia in rats with chronic stress depression is more effective.Conclusion:1. Inside EA Neiguan by reducing Gi protein expression and protein expression increa sed Gs, to protect cardiomyocytes from ECG improvement, reflected in improved tissu e morphology.2. Acupuncture C57BL/6 mice were significantly better than the therapeutic effect of ASIC3-/- mice, a possible mechanism is the afferent nerve receptors involved in the m echanism of acupuncture effect ASIC3 outer periphery, so acupuncture Neiguan can be activated by ASIC3 body ion channels, thereby inhibiting sympathetic to ease due to myocardial sympathomimetic isoproterenol induced ischemic symptoms.3. Chronic Stress Depression increased myocardial ischemia, indicating emotional factor s can alter the development of myocardial ischemia by inflammatory cytokines. Electro-acupuncture of Neiguan can to relieve myocardial ischemia in patients with chronic d epression rat myocardial injury by regulating the expression of inflammatory cytokines and myocardial cells Gi, Gs protein, thereby simultaneously treating emotional disease and heart disease, is described Neiguan emotion with heart disease interrelated meridi an foundation. |
PDF Full Text Request