| This article extract flavonoids in apples, extraction process, separation and purification technology, research structural studies of flavonoids and anti-lipid oxidation aspects of traditional security risks grease antioxidant BHT and flavonoids rutin oil antioxidant substances on behalf of the ability to compare, to find new anti-oxidant oils Apple added value and improve the theoretical foundation.Apple fruit is rich in polyphenols, after the destruction of the cells, polyphenol oxidase can quickly destroy flavonoids has physiological functions in apples, and therefore raw material pretreatment, using100℃heating destroyed more than short-term phenol oxidase to protect flavonoids. After pretreatment of raw materials, the use of single-factor test and orthogonal experiment method to determine the organic solvent extraction process of hot-dip flavonoids, extraction temperature is70℃, extraction time was1hour,75%ethanol concentration, solid-liquid ratio1:10, have to flavonoids extract each sample was two times,extracting solution is collected,the extract was evaporated concentration of alcohol precipitation process.By static adsorption and analytical method, the macroporus resin was screened by Langmuir equations, the separation and purification of flavonoids from apple is finally selected macroporous adsorption resin AB-8. The single factor experiment and two general revolving combination design and optimization of AB-8macroporous resin separation and purification of Apple flavonoids: every time the sample volume is200.00mg flavonoid content on250mL, eluent ethanol concentration80%,1.5mL/min of sample solution flow rate, eluent flow rateis1.8mL/min of sample solution, the concentration of0.82mg/mL, resinused every2cycles for a regeneration, the yield rate of flavonoids was84.03%on average, collect the eluent, concentration and drying, the crude flavonoids was measured by using aluminum salt complexing chromogenic method,in the follow-up study found that other magazines interfere the determination of purity. The crude mixture of flavone compounds, use dextran gel Sephadex LH-20column for further separation of the monomers, separation and purification of the crude flavones was added1mL gel column, eluted with a gradient flow rate of0.8mL/min, eluent methanol solution after completion eluting with a gradient of pure methanol until HPLC detected straight stop. Combined with HPLC detection of the eluent can successfully get a flavonoid monomers, using high-performance liquid chromatography detection of purity flavonoids monomeric is83.579%.Apple flavonod monomers,research structure and antioxidant function.Flavonoid compounds containing functional groups are special,and theoxidantion or reduction ability of different compounds react,cause color change.According to the change of the color that flavonoid monomers as flavonols.Using the characteristic UV spectra of flavones,displacement agent added observed UV spectrum,conclusion flavonoids substituent,determine the flavones nucleus3positon with hydroxyl substituent, determine the flavones nucleus3position with hydroxyl substituted,around4′and7adjacent two position of phenolic hydroxyl group.Using the oven method to detect anti-lipid oxidation Apple flavonoid compounds were rutin, BHT, the antioxidant effect of BHT> Apple flavonoid compounds were> rutin. From the structural analysis of Apple only antioxidant flavonoids monomer A ring is higher than rutin; while3-OH C ring is replaced rutinose rutin, apple flavonoids monomer has3-OH, can be combined with four carboxyl chelate metal ions to form stable, extended oil autoxidation induction period. Apple flavonoids both health functions, there are better antioxidant capacity, consider adding special health supplements. |
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