Research On Technology Of Applying Macroporous Adsorption Resin To Separate Flavonoids Of Herbs Process And Design Of The Production Line

D101macroporous adsorption resin was chosen in present work to study the effect of resin regeneration method and technological conditions on separation of flavonoids of Rhizoma Drynariae, Carthamus tinctorius L. and Ginkgo biloba leaves. Macroporous resin was regenerated by combing short-term regeneration and the long-term regeneration. According to optimized macroporous resin adsorption technological conditions of the three herbs, the stability and feasibility of applying macroporous resin regenerated multi-times were evaluated. In order to prepare flavonoids on industrial equipmemt, a plant scale production line based on configuration technology of macroporous resin was designed in a pharmaceutical factory.The methods of detecting the content of total flavonoids were established based on UV spectrophotometry.HPLC was applied to build test methods of the content of naringin, hydroxysafflor yellow A and the flavonol glycosides of Ginkgo folium. Basing on the static and dynamic adsorption test of the macroporous resin, adsorption capacity, adsorption rate, parse rate and eluting ability were used to optimize mircoporous resin adsorption technology for purifying flavonoids of Rhizoma Drynariae, Carthamus tinctorius L. and Ginkgo folium.The quality of flavonoids extracted from the herbs by using macroporous resin regenerated multi-times was investigated. The results indicated that the average content of total flavonoids and naringin of nine batches of extracts of Rhizoma Drynariae, the average content of total carthamin yellow and HSYA of nine batches of extracts of Carthamus tinctorius L. and the average content of total flavonol glycosides of eleven batches of extacts of Ginkgo biloba leaves changed a little. The RSD of the contents were in the range of1.98%～7.69%. It showed that combing multi-times short-time resin regeneration and few times long-term resin regeneration could effectively separate the flavonoids from the three herbs and reduce the cost of regenerating resin and operation time.The research results of present work provided foundamental data for industrial application of using macroporous resin adsorption process to separate flavonoids from herbs. The designed plant production line of macorporous resin adsorption technology was installed smoothly. It laid the fundationof for promotion of the process in the industrialization production.