Studies On Preparation And Photo Responsiveness Of Sasanquasapon In Nanocapsules

The saponin is a kind of oleanane-type pentacyclic triterpenoid mixture presentin seeds of tea plant or Camellia oleifera. The saponin is composed of three partswhich are sapogenin, glycosides and organic acids. But it absorbs moisture easily,hydrolyzes easily and can’t preserve well, limiting its application. However, itsstability can be improved after acid hydrolysis. In the meantime, after encapsuling,the medicine will become more stable and effective. Recent studies show thatnanoparticles can penetrate the blood-brain barrier, improve the bioavailability,enhance targeting and control release etc. In the preparation of nanocapsule, Camelliasaponin and Camellia saponin acid hydrolysate are used as capsule core, soybeanlecithin and cholesterol are used as capsule shell. The main experimental methods andresults are as follows:(1)Dissolve the capsule core in pH=7.5phosphate buffer which contains Tween-80, and dissolve the capsule shell in ethanol at60℃. Then drop the capsule shellslowly into the capsule core solution in water bath under60℃, reacting30min. Theparticle size of the Camellia saponin nanocapsule was63.97nm, the zeta potential was-58.2mV, and the entrapment efficiency was74.06%. The particle size of Camelliasaponin acid hydrolysis nanocapsule was155.5nm, the zeta potential was-51.9mV,and the entrapment efficiency was65.25%.(2)Select the core content, the capsule material proportion and reaction time asthree factors, each with five levels, to screen the best preparation method,usingparticle size, zeta potential, entrapment efficiency as detection index. The optimalpreparation method of Camellia saponin nanocapsule was: core content0.025g,capsule material ratio of lecithin vs cholesterol=5:1, reaction time30min; and theoptimal preparation method of Camellia saponin acid hydrolysate nanocapsule was:core content0.035g, capsule material ratio of lecithin vs cholesterol=1:1, reactiontime15min.(3)Add different doses of pheophorbide a in the capsule shell preparation processand illuminate30minutes. The addition of pheophorbide a influenced on the size,zeta potential and encapsulation efficiency of two kinds of nanocapsules slightly.Andpheophorbide a significantly increased the accumulated release of Camellia saponinnanocapsule within12hours, but not the Camellia saponin hydrolysate of nanocapsule. Therefore, pheophorbide a mainly increased the Camellia saponinnanocapsule accumulated release smoothly and efficiently.