Purification Research Of Cordycepin By Macroporous Adsorption Resin

Choosing the right resin is the key of cordycepin purification. Based on four kinds of macroporous adsorption resins HPD722, HPD750, AB-8, RESIN-Ⅱstatic adsorption and desorption characteristics of cordycepin, choosing RESIN-Ⅱ resin used for extraction of cordycepin Finally. Static adsorption of macroporous resin RESIN-Ⅱ can achieve good effect in6h, and the balance of desorption needs120min with50%ethanol.The factors that impact macroporous resin RESIN-Ⅱ on the properties of cordycepin adsorption have pH and velocity. The best adsorption conditions are pH9, Adsorption velocity of2BV/h and macroporous resin NKA-Ⅱ of cordycepin adsorption capacity can reach16.5mg/g. The factors that impact macroporous resin RESIN-Ⅱ on the properties of cordycepin desorption have pH, ethanol volume fraction and velocity. The best desorption conditions are pH9-pH12,50%ethanol elution, flow rate of3BV/h, and macroporous resin NKA-Ⅱ of cordycepin desorption can reach above95%.Comparison of pre-alcohol precipitation and post-alcohol precipitation in cordycepin extraction process. The experiments show that post-alcohol precipitation can save a lot of ethanol; Although cordycepin yield of pre-alcohol precipitation is higher, cordycepin precipited after post-alcohol precipitation also can be recycled to purification. Therefore, in fact The loss of cordycepin was not much higher than pre-alcohol precipitation. In order to better development of cordyceps militaris resources, and reduce the dosage of ethanol, it’s necessary to Separate and purify cordycepin that exit in Artificial cordyceps militaris culture medium residue. The optimum conditions of post-alcohol precipitation: condense the eluent20times, make the final concentration to80%with anhydrous ethanol and place9h, Supernatant standby after centrifugal; Take impurities dissolved with80%ethal per gram2ml, take supernate after centrifugal Separated and purified again.Comparison of Extraction of cordycepin by macroporous adsorption resin RESIN-II and CATION cation resin, ANION ANION resin combination. The results show that both methods can be used to make cordycepin crystal. The macroporous resin RESIN-II has a large adsorbance and a high desorption, but the ability of separating pigment is poor. The CATION and ANION resins combination can obtain more cordycepin crystal, but it has a poor adsorbance and desorption.