The Synthesis And Application Of A Fluorescent Probe For Zn (Ⅱ), Cu (Ⅱ) Relay Recognition Based On N,N-bis(2-pyridylmethyl)Amine

Zinc and copper are of significant importance in nature. In addition to itsapplication in engineering, its role played in the existing and development oforganism is also irreplaceable. Detecting existing modes and contents of zinc andcopper is becoming more and more important.Small organic molecular probe is prevalent for its easy preparation, high selectivityand sensitivity, low detection limits. Multifunction enables the dual-recognition probe,a new category, to detect more than one substance serving us through cutting the costand working more efficiently. This paper details the optical properties, bindingbehaviors, sensing mechanism and sample detection of a novel dual-recognitionsensor, entitled NBPA, based on N,N-bis(2-pyridylmethyl)amine.In the first part, we discussed the research background, including the significance,methods of detecting zinc and copper, category of binding sites of zinc or coppersensors, classification of dual-recognition sensors.In the second part, we explained the synthesis route of NBPA as well as itscharacterization by mass spectra and NMR.In the third part, we studied the selectivity of NBPA, optimized the experimentalconditions and applied it into real samples determination. Under optimized conditions,the fluorescence intensity is linear to the concentration of Zn2+and Cu2+separatelyexhibiting good linearities from1.0×10-6M to8.0×10-6M and1.0×10-6M to1.0×10-5M (R2=0.9996and R2=0.9914) with detection limits of7.89×10-7M and1.27×10-7M respectively. The proposed sensing mechanism has been proved by Job’sPlot, mass spectra, NMR titration, which would be beneficial to studies in the future.