Study On Biological Saponin Removal Of Camellia Oleifera Seed Cake

The Camellia scraps is byproducts of tea seed after oil pressing, and they are different by various methods. Output by physical squeezing called Camellia scraps of "cake", while manufactured by chemical extracting method called "meal". Tea meal has extensive nutrients, such as crude protein, cellulose, mineral elements, etc. But the tea meal contains a certain amount of tea saponin, which is hindered utilization of tea meal because of its hemolysis and ichthyotoxin. If we can break through the bottleneck, making saponin degradation of biological fermentation can get efficient use of rich seed meal resources, not only saves resources but also protect the environment, and create economic value for the country.1.In this paper, through screening high efficient degradation strains of tea saponin to achieve the goal of biodegradation or detoxification, and to provide theoretical basis and pratical basis for the tea meal used in amino acid foliar fertilizer. Those strains’degradation rate reached68.5percent by the vanillin sulfuric acid method for the determination, as tea saponin biomass reached8.9×109cfu/ml. After comparison, they turn out to be the Bacillus amyloliquefaciens.2.Optimizing of fermentation condition and determining of the optimal media formulations are made in laboratory levels (shaking flask) to saponin degrading strain N-1. And the best medium formulation is that6percents tea meal,1percent soluble starch,0.2percent potassium dihydrogen phosphate,0.05percent calcium chloride as well as magnesium chloride,0.5percent sodium chloride. Meanwhile, optimization results are that temperature is36Celsius degree, rotation speed is160round per minute, incubation time is60hour, and pH7.0.3.Small and medium type of research show the results of50litre level optimizationt are that the6percent concentration of tea meal,8percent of inoculum size,140round per minutes of stirring rate,1.6cubic meter per hour of ventilation volume. And the results of500litre condition are as follow:8cubic meter per hour of ventilation volume,0.02MPa of cans pressure,160round per minutes of stirring rate. Under this500litre fermented condition, the tea saponin degradation rate reaches65.8percent, and biomass achieve12×109cfu/m. The main nutrients of tea saponin degradation rate is61.3percent, crude protein is1.2percent, and the content of free amino acid in fermentation broth is38milligram per gram.