| In recent years,China's the Camelliaoleifera economic is developing rapidly,Camelliaoleifera planting area is expanding increasingly, the current development andutilization of Camelliaoleifera processing are mainly concentrating in tea-seed-oil,while after extracting oil the Camelliaoleifera cake is leaving as waste, therefore,Camelliaoleifera cake is rich in resources,and the research of tea saponin inCamelliaoleifera cake, strengthen its application in other sectors to achieve the purposeof turning waste into treasure, bringing enormous economic benefits, social benefitsand ecological benefits. This paper use ethanol crudely to extract tea saponin fromCamelliaoleifera cake, analysing various factors on the impact of tea saponin, thenusing precipitation and adsorptive resin method to purify tea saponin, and finding outthe method is suitable for industrial production of tea saponin. Systemly researching thetea saponin in different bacteria and fungi inhibiting ability, also studying theemulsifying characteristics of tea saponin, Analysising the foam capacity, surfacetension and contact angle of tea saponin. Finally, this paper also studies the differentformulations of washing products, through the washing efficiency and residual oilextraction aspect comparison, pick out in accordance with the production of detergentformulations, for the future research and development foundation.(1)Using organic extraction,and doing coarse extraction from tea saponin by Ethanol.By single factor experiment study the influence of ethanol volume fraction,extractiontime, extraction temperature, ratio of solid to liquid on tea saponin yield. Through theorthogonal experiment Optimize tea saponin extraction process,then getting theoptimum leaching solution.The results show that:during the extraction progress,ethanolvolume fraction,extraction time, extraction temperature, ratio of solid to liquid are allInfluence yield of tea saponin. Yield of tea saponin is influenced most by ethanolvolume fraction, followed by extraction time and extraction temperature,while ratio ofsolid to liquid has the lest influence. The optimum technology for the extraction of teasaponin is A1B2C1D2,which is volume fraction of ethanol is80%, extraction time is2hours, the extraction temperature of75DEG C, solid to liquid ratio is1:10.(2)Drawing standard curve of tea saponin, through precipitation and macroporous resinadsorption purify tea saponin samples, calculating the teasaponin-content of purifiedsample. The results show that: different macroporous resin has different adsorption rateof tea saponin,AB-8macroporous resin has the highest adsorption rate of tea saponin, DM-301macroporous resin's adsorption rate is66.21%, NKA-Ⅱmacroporous resin'sadsorption rate is53.47%.Purifying tea saponin by AB-8macroporous adsorptive resin,tea saponin content of sample is80.55%; purifying tea saponin by precipitation, teasaponin content of sample is73.59%. As a result of macroporous adsorption resin hasrenewability, so can be used in industrial production to reduce costs.(3)Using filter paper antibacterial experiment researching tea saponin's inhibition onEscherichia coli, Yeast, and Aspergillus niger. The results show that: tea saponin againstdifferent microorganisms with different degrees of inhibition, different concentrationsof tea saponin solution has obvious regularity effect, along with tea saponin solutionconcentration increasing, the antibacterial circle diameter is on the increase,andshowing the obvious concentration dependence. Tea saponin has the strongestantibacterial activity on single cell fungal yeast;has the weakest inhibitory ability onspores streptomyces, only to the degree of inhibition,and the concentrationdependence is not obvious.(4)Utilizing tea saponin foam capacity experiment,surface tension experiment,contactangle experiment to study surface activity and emulsification characteristics of teasaponin.The results show that:tea saponin could obviously increase the beer foamingtime,and along with the increasing concentration of tea saponin,beer foam capacity alsoincreased significantly. Tea saponin could significantly reduce the surface activity asthe concentration increasing. When the concentration is0.05g/ml, the surface activityis lowest. Then increasing the concentration of, the activity no longer reducing.Presenting the peculiar surfactant micelle formation phenomenon. Tea saponin couldsignificantly reduce the contact angle of water, and with the tea saponin concentrationincreasing,and contact angleθ﹤90°. Contact angle usually reflects surfactantwettability. The contact angle is smaller, the wettability is better.So tea saponin can beused as an excellent surfactant.(5)Preparing detergent samples with different formulations, and using washing oil ratedetermination(QBT2117-1995universal water-based metal cleaning agent),woolscouring experiment and residual oil extraction(GB6977-86washing wool oil, dust,soil miscellaneous test method), determinating the washing effect of detergent withdifferent formulations, understanding the advantages and disadvantages of detergentwith tea saponin. The results show that: in washing oil rate determination of detergentexperiment,The washing oil rate of tea saponin to rapeseed oil is not high. |
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