| Peanuts testa, which usually be used as by-product in peanut industry, have several polyphenol compound materials with important physiological function such as Resveratrol(Res.), Tannic Acid(TA), Procyanidins, Flavones. So it is highly profitable and profoundly remarkable for the natural active component extraction and purification from peanut testa.Research had been carried out on the content of several polyphenols in peanut testa; the yields of Procyanidins, Res., Flavone, GA, were 11.26%,0.0216%,2.59%,10.03%, respectively. Based on the single factor experiments, the extraction conditions were as follows:the ratio of solid to solution is 1:16, the concentration of ethanol is 80%, extraction time 10 min,240W of ultrasonic power.Research had been made on the technology of puring PTE with AB-8 macroporous adsorption resin by static adsorption and de-adsorption tests. The single factor tests had been applied to optimize the enrichment and separation conditions of PTE. The best adsorption conditions were:PTE concentration is 4mg/mL, feeding rate is 1.0mg/mL, pH has no remarkable effect. The optimum elution conditions were 40% ethanol, eluent volumes was 120mL. The products can be obtained by concentration and lyophilization, the contents of PTE was 68.40%.With the experiment of DPPH scavenging hydroxyl radicals, reducing power, inducing time of lard, the antioxidant activitives of PPTE had been investigated. Results showed that the antioxidant ability of PPTE is higher than VC by the method of reducing power; by 734 Rancimat method determination results of the oxidative stability of lard showed that the antioxidant ability of PPTE is up to the highest when the concentration is 150 mg/L and at the same concentration compared with controls, longer than Vc, GA; but shorter than BHT.PPTE has strong inhibitory effects on Staphylococcus aureus, Escherichia coli, Penicillium sp., Actinomucor sp. and Aspergillus niger. The minimum inhibiting concentrations are all 250mg/L for all tested microbe. |
PDF Full Text Request