Determination Of Tetramethrin And P-nitroaniline, M-nitroaniline By Liquid Microextraction Capillary Electrophoresis

In recent years, the capillary electrophoresis method has been widely adopted in the field of analytical chemistry for its fast analysis speed, less sample volume, high sensitivity and simple operation by instrument. At present, the combination of capillary electrophoresis with other various analysis methods are also widely applied, such as capillary electrophoresis electrochemiluminescence assay(CE-ECL),capillary electrophoresis ultraviolet spectrophotometry(CE-UV) and capillary electrophoresis mass spectrometry(CE-MS)etc. These new combination methods greatly expand the application range of capillary electrophoresis and also significantly improve the detectability of samples.This thesis mainly analyzes the determination of tetramethrin in water by capillary electrophoresis with chemi-luminescence and the determination of p, m-nitroaniline by capillary electrophoresis with PDA spectrophotometry, respectively. The optimum determination conditions of tetramethrin and p, m-nitroaniline are determined,together with influence factors including types of buffer solutions,the value of p H, the separation voltage and the chemiluminescence intensity.In the experiment, influence factors such as extraction time, p H value of buffer solution, separation voltage, capillary electrophoresis, chemiluminescence intensity and others are carefully compared and tested, and the optimal experimental conditions for determination of tetramethrin by capillary electrophoresis with chemiluminescence are obtained at last.Due to the similarity in chemical properties and spatial structures between p-nitroaniline and m- nitroaniline, simultaneous determination of both substances is difficult to realize. However, these two substances are determined at the same time by capillary electrophoresis in this thesis. The optimal separation and experimental conditions for the determination of m-nitroaniline and p–nitroaniline are obtained, including types of buffer solutions, p H, the separation voltage and the separation time.