Study On Identification And Activity Of Flavonoids In Natural Honeycomb

Material is Honeycomb in this paper. Bioactive ingredients of Honeycomb were analyzed systematic. Extraction conditions of Flavonoids in Honeycomb were analyzed through Matrix Solid Phase Dispersion. Method for determination of High Performance Liquid Chromatography.Further separation and purification and then its structure was analyzed. Antioxidant and antibacterial activity of Flavonoids in Honeycomb were studied with the following results:1. Direct titration method, Kjeldahl nitrogen method contained three, indene ketone spectrophotometry, ultraviolet spectrophotometry and other chemical methods respectively in the honeycomb total sugar, protein, amino acids, organic acids were analyzed.2. Three kinds of methods for extraction of Flavonoids from the analysis and comparison of Honeycomb, the optimum extraction method and matrix solid-phase dispersion method to determine. Based on the dispersing agent, leaching agent, leaching agent amount, eluent and elution of single factor test agent that the optimum extracting conditions of flavonoids in Honeycomb as:silica gel as dispersing agent,20mL hexane as eluent,20mL methanol as eluent, the extraction rate of flavonoids in this condition for 5.4mg/g.3. HPLC was used for the qualitative and quantitative analysis of flavonoids in the extraction of honeycomb, honeycomb flavonoids obtained under condition of 0.2% aqueous solution of phosphoric acid acetonitrile gradient elution rate of rutin 12.68ug/g, Morin 137.72 ug/g, quercetin 23.07 ug/g, luteolin 76.34 ug/g, apigenin 21.09 ug/g,chrysin 331.98 ug/g, kaempferol 291.64 ug/g.4. Purification of honeycomb of flavonoids extraction with D-101 macroporous resin, using wet column packing, get the best conditions for purification of:at a flow rate of 1mL/min sample 30mL of sample solution, fully absorb, using 65% ethanol as eluent, at a flow rate of 1mL/min for elution.5. Determined by UV spectrophotometer, high performance liquid chromatography, infrared spectroscopy, mass spectrometry detection analysis, to extract honeycomb flavonoids results show that:the sample properties and structure and flavonoids extraction agreement standard.6. Using in vitro antioxidant models of honeycomb flavone extract antioxidant activity were studied with different concentrations of vitamin C as the control, clearance rates were studied to extract honeycomb flavonoids on DPPH, superoxide anion free radical, hydroxyl radical, find honeycomb flavone extract has stronger antioxidant ability.7. Study on the antibacterial activity of flavonoids extract from the honeycomb, experiments show that honeycomb flavonoids extracts on actinomycetes, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, beer yeast has a good bacteriostatic effect. The minimum inhibitory concentration of each species were 25mg/mL,12.5mg/mL,6.2mg/mL, 6.2mg/mL,3.1mg/mL.