Study On The Improvement Of Mice Intestinal Microecology By Lentinan Combined With Probiotics And Product Development

The topic is a joint development project about the Institute of Agro-Food Technology, Jilin Academy of Agricultural Sciences with the fungus corporation named Shenyi of Hunchun province, researching and providing production technology and related functional verification about high-end features of Shiitake product for corporation. Shiitake is a kind of medicinal and edible fungus, which is famous and precious in the world. Lactobacillus plantarum C88 is a strain of lactic acid bacteria, which is isolated and identified from traditional fermented foods.Based on the functions of improving bowel and regulating immunity of L. plantarum C88 and lentinan, this study will be conducted in the extraction and purification of lentinan experiments, testing its content of polysaccharide and purification rate. to this paper will study the combination of lentinan and L. plantarum C88 to improve bowel function on mice through animal experiments, which is also providing a theoretical basis for the development of lentinan and L. plantarum C88 product. Finally, this paper reasearches on initial development composite products of lentinan and L. plantarum C88.(the chewable composite of lentinan and L.plantarum C88). The Experimental results are as follows:(1) The cream rate of fresh shiitake and dried shiitake was 4.91% and 24.9%; the content of lentinan from fresh shiitake and dried shiitake was 50.29% and 44.14%. The purification of crude lentinan, the mass fraction of neutral and acidic lentinan was 45%and 31%, the content of lentinan was 88.15% and 66.9% respectively. In prebiotic activity assay, the results show that neutral lentinan and acidic lentinan have some prebiotic effects, compared to other strains, L.plantarum C88 has highest growth rates, which can use neutral lentinan and acidic lentinan effectively, and the effect of neutral lentinan prebiotic is stronger than acidic lentinan.(2) The combined utiization of lentinan and L. plantarum C88 can effectively improve the intestinal flora in mice, maintain intestinal health, it is better than used lentinan or L. plantarum C88 only. Different doses of lentinan combinate with L. plantarum C88 can significantly reduce serum DAO, D- lactic acid content, maintain the integrity and permeability of the intestinal mucosa, and intestinal barrier function; reduce toxin levels and inflammation in thebody factor levels. Remarkably promote the growth of Lactobacillus, Bifidobacterium,inhibited the growth of Enterobacilli, Enterococcus in intestine of mice. Growth of beneficial bacteria in the gut of mice can increase the content of short-chain fatty acids, improve the content of acetic acid, propionic acid and butyric acid in intestine respectively, by 1.51 mg/m L,0.12 mg/m L and 0.15 mg/m L up to 2.16 mg/m L, 0.20 mg/m L and 0.26 mg/m L; the value of p H in feces decreased from 7.350 to 7.023; the content of moisture in feces reduced from 63.81%to 50.74%; the content of ammonia in feces reduced from 66.86 μg/g to 52.45 μg/g significantly.(3) The optimal process parameters: ethanol concentration at 70%, dryi temperature at70℃, dryi time at 30 min. The final optimized formulation of the main raw material of the product: the dosage of shiitake citric acidextract, ratio of sucrose, FOS and planting the fat end is 25%, 10:1, 10% and 3%. Sensory analysis: the color of chewable is light brown and the chewable has a unique flavor of shiitake. Physical and chemical analysis: tablet weight is 0.5 g,slice thickness is 3.0 mm, the water content is 1.4%, moderate hardness chewable tablets is 4.5kg/mm2, homogeneous, smooth surface, and has a higher survival rate of probiotics.The combined utiization of lentinan and L. plantarum C88 can improve the bowel function in mice by experimental validation, including gut microflora, intestinal permeability and integrity, the content of fecal ammonia, the value of p H, short chain fatty acid and other indicators. Finally successfully developed a functional health products of complex chewable tablet for Shenyi mushroom company, providing the production technology and theoretical basis of mushroom products of development and utilization, but also opening up a new direction of development for the market of polysaccharide and probiotic.