Chemiluminescence Study Of The New System And Its Applications In Pharmaceutical Analysis

Abstract Tow parts, review and research report, are included in this thesis.In the first part, the new development in drug-pfOtein binding in recent years isreviewed. Drug-protein binding is an importan process in determining the acivity andfate of a pharmaceutical agent once it has entered the body This review includes thetheory of drug-protein biriding, several separatbo procedures and their applications indrug-protein binding.In the second ped, the research report is focosed on the applications of several newchemiluminescence systems in drug analysisl. Fe(CN)₆³ Chemiluminescence system for RutinA novel chemiluminescence(CL) System for Rutin combined with flow injecionanalysis is presented in this paper. It is based on the CL reacion of Rutin andhexacyanoferrate(III) in sodium hydroxide medium. The proposed method has a Iinearrange of lxl0⁴g/mL ～ lxl0⁶g/mL with a RSD of 3.7%(5x1O^-6g/mL, n=11) and asample throughput of 60 h^-l. The method was successfully applied to the determinationof Rutin in a pharmaceutical preparation.2. Flow-injection system for automated dissolution testing of isoniazid tablets withchemilumiescence detertionA simple and sensitive flow-injection chemilumiescence(cL) system for automaeddissolution testing is described and evaluated for menitoring of dissoforion profiles ofisoniazid tablets. The undissolved suspended particles in the dissolved solution wereeliminated via on-line filter. The novel CL system of KIO₄-isoniazid was alsoinvestigated. The sampling frequency of the system was l20 h^-l. The dissolutionprofiles of isoniazid fast-release tablets from three sources were determined, whichdemonstrates the stability, great sensitivity, large dynamic measuting range androbustness of the system.1 A Chemiluminescence Method fOr the Oetermination or Rifampicinumln this paPer, the now-injection chemiiuminescence (cL) determination ofRifampicinum was suggested. It was based on the CL reaction of hydrogen peroxideand rifampicinum in presence of Cu'+, as cataIyst, in basic medium. The CL intensitywas linear with rifampicinum concentration in the range of 4 X l0-7 l X l0'g/mL: Therelative standard deviations for 2X l0-5 g/mL rifampicinum (n=7) was 4.9O/o.ThedeteCtion limit was 7 X l0-' g/mL. This method was used to determine rifampicinum inrifampicini ocustilla with satisfactoty results.4. Flow-iulection chemiluminescence det.rmination of chIortetracycIine using'on-line electrogenerated lCu(HIO.),l'- as the oxldafftA novel chemiluminescence (CL) flow system fOr the determination ofchlortetracycline is described. It is based on the direct CL reaction of chlortetracycIineand [Cu(HIO.),]'- in KOH medium. The unstable ICu(HIO.),]'- was on-lineeleCtrogenerated by constant-current eIectrolysis. The CL intensity was Iinear withchlortetracycline concentration in the range of 0. I^l00 ndml. The determination Iimitwas 5.3x10' g/ml. The whole process cou1d be completed in l min. The proposedmethod is suitable for auomatic and continuous analysis, and has been aPpliedsatisfactOrly to analysis ofchIortetracycline in biolo8ical nuid.