Research Of Physiological Property, Tissue Culture And Rapid Propagation Technology On A New Variety Of Lonicera Japonica Thunb. 'yihao'

Objective:To study on photosynthetic characteristics, tissue culture and rapid propagation technology on Lonicera Japonica Thunb.'Yihao' and supply theory evidences and operative technologies for establishment of standard operating procedure of planting and popularity in production.Method:CIRAS-1 portable photosynthesis measuring instrument was utilized to analyze the physiological indexes such as photosynthetic rate, etc; The development of callus and conditions for induction of adventitious bud and root-producing culture were explored with combination of MS and hormone in different proportions as culture medium.Results:The light saturation point and compensation point of Lonicera Japonica Thunb.'Yihao'were 1529μmol/m2/s and 48μmol/m2/s respectively. Different sterilizing effects were obtained with different disinfectants; Distinct effects of gathering time of explant on tissue contamination and sterilization time on tissue browning were gained. The development of callus, induction of adventitious bud and root-producing culture varied largely with different hormone proportions.Conlusions:A new variety,Lonicera Japonica Thunb.'yihao'is a typical heliophyte that its planting must be carried out under ample sunlight. Robust young and soft terminal buds or stems with axillary buds could be gathered during germination in March to May and inoculated after disposal with 75% ethanol for 1min and 0.1% mercury for 5-6min that low contamination rate and browning rate could be obtained. The best first culture medium for induction of adventitious bud was combination of MS,6-BA 1.5mg/L and NAA 0.05mg/L. The best successive culture medium for propa gating was combination of MS,6-BA 0.5mg/L and NAA 0.05mg/L. Other optimal culture mediums were obtained as follows:MS+NAA0.lmg/L for root producing; MS+6-BA0.25mg/L+KT0.5mg/L+ NAA0.1mg/L for white callus; MS+6-BA1.0mg/L+KT0.25mg/L+NAA0.5mg/L and MS+6-BA 2.0mg/L+KT0.5mg/L+NAA1.5mg/L for induction of green callus; MS+ 6-BA 0.5mg/L+NAA 0.02mg/L for callus differentiation and proliferation. The study shows that terminal bud is the optimal explant resource for rapid propagation of callus.