Use Of Suppression Subtractive Hybridization Beet Apomixes Expressed By Flowering Gene Research

A set of monosomic addition lines of Beta corolliflora were the progenies from the hybridization of B.vulgaris L. and B.corolliflora Zoss in sugar beet .Among these monosomic addition lines,a line,designated as Mu, that constituted of the normal 18 B.vulgaris chromosomes with the NO.9 chromosome of B. corolliflora ,was found having a chromosome transmission frequency 97.5%;while the other lines with different extra chromosomes from B.corolliflora were at chromosome transmission frequencies less than 50%.The reason that the transmission frequency in Mm was so hign was interpreted as a possible occurrence of apomixis in the NO.9 chromosome of B.corolliflora in M14.In order to isolate and identify the the apomixis gene(s) of M|4,the differential expression clones had been obtained by the method of SSH (suppression subtractive hybridization )and later had all been sequenced to become ESTs.These ESTs in theory were only expressed in Mi4,but were not expressed in A2Y.We used them to screen the non-redundant GenBank of NCBI by Blast programe.The Blast similarity search showed that 121 ESTs represented the part of the known genes,90 ESTs represented the part of the new genes.From them we can select the ESTs related to the apomixis gene(s),which can futher be used to clone the whole length of the apomixis gene(s).