Microsatellite Marker Polymorphism And Silkies Slaughter Performance Of The Analysis

Fifteen microsatellite markers in Chrl and five microsatellite markers in Chr Z selected for multiplex PCR in silkie, the production were detected automatically on ABI3100.Allele number average heterozygosity(H) polymorphism information content (PIC) and allele frequencies were calculated by Cervus2. 0. The GLM between markers and thirteen dressing characters was performed by SAS6.12. To cut down the workload and the cost, we optimized the conditions of the multiplex PCR and the combination of the markers. The results indicate:(1) After the conditions of the multiplex PCR and the combination of the markers optimized many times, 5 sets were filtrated successfully Which could be detected in 3 sets using ABI3100-Avant. It indicated that this experiment of multiplex PCR sets was successful.(2) 18 pairs of microsatellites were polymorphism. The average number of alleles detected by a pair of primer was 6.83. The average PIC was 0.805. And the population heterozygosity was 0.833.(3) We found there were significant correlations among 13 markers and 13 characters through GLM.There is significant correlation between LEI0146 and the Live Weight.MCW0154 LEI0134 and LEI0146 are all remarkably correlative to the Eviscerated weight with giblet. Among those, the correlation between LEI0146 and the Eviscerated weight with giblet is extremely significant. Also, LEI0146 is remarkably correlative to the claw weight. The correlation between the Heart weight and LEI0146 is significant while it between the heart and MCW0036 is extremely significant. LEI0146 and MCW0200 are allextremely correlative to Liver weight. The correlation between MCW0313 LEI0134 MCW0331 and the Ventriculus muscular is weight is significant. MCW0254 LEI0134 are all remarkable correlative to the Breast muscle yield weight and the Leg muscle yield weight, notably, the correlation between MCW0254 and the Leg muscle yield weight is extremely significant. Which are remarkably correlative to the Eviscerated weight are MCW0154 LEI0134 and LEIO146, among those, the correlation between LEI0146 and the Eviscerated weight is extremely significant.MCW0168 MCW0331 LEI0106 MCW0112 are all remarkably correlative to the Percentage of eviscerated yield while the correlations between MCW0258 and LEI0146 are remarkably significant. About the Percentage of eviscerated yield with giblet, it is significantly correlative to MCW0331 LEI0106 MCW0112 and the correlation between the Percentage of eviscerated yield with giblet and MCW0258 LEI0146 is extremely significant. MCW0258 and MCW0200 are significantly correlative to the Percentage of breast muscle yield, and the correlation between MCW0200 and the Percentage of breast muscle yield is extremely significant. MCW0177 and MCW0258 are all remarkably correlative to the Percentage of leg muscle yield, and the correlation between MCW0258 and the Percentage of leg muscle yield is extremely significant.