Of Cd (2 +) Induced Change Of Vegetables, Different Organizations Proteome Research

In this study, four vegetable cultivars, cucumber (cucumis sativus. L.), tomato (Lycopersicon esculentum L.), radish ( Raphanus sativus cultivar L.) and Chinese cabbage (Brassica campestris L.ssp. pekinesis) were used as experiment materials. Changes of growth characteristics (height of seedlings, length of roots and fresh, dry weight) and biochemical characteristics (chlorophyll content and protein composition) were studied in vegetables seedlings exposed to different concentrations Cd²⁺ by experiment.The experiment result showed that a positive relationship was observed between the inhibition of seedling growth and changes in biochemical characteristics in the four vegetable cultivars. The root of cucumber seedling was the most sensitive to Cd²⁺ treatment. Root length was decreased from 5.85±1.09 cm (in 1 mM Cd²⁺ treatment) to 1.09±0.31 cm (in control). Tomato was also sensitive to Cd²⁺ treatment. Seedling height was decreased from 4.76±0.5 cm (in control) to 3.79±0.50 cm (in 0.03 mM Cd²⁺ treatment). Similar experiment results were observed in fresh weight analysis in four vegetable cultivars, but the change in dry weight wasn't obvious compared with the control. In addition, chlorophyll contents decreased gradually with Cd²⁺ concentration ranging from0 to 1 mM.Representative silver-stained 2D polyacrylamide gel analyses indicated changes of the root, plumular axis of tomato, radish, Chinese cabbage seedlings (treated with 0.05 mM Cd²⁺) and cucumber seedlings (treated with 1 mM Cd²⁺). 18 protein spots, including 8 protein spots in root, 10 protein spots in plumular axis, were altered in tomato cultivars. 43 protein spots, including 19 protein spots in root, 24 protein spots in plumular axis, were altered in radish cultivars. 45 protein spots, including 25 protein spots in root, 20 protein spots in plumular axis, were altered in Chinese cabbage. 26 protein spot, including 8 protein spots in root, 3 protein spots in plumular axis, 15 protein spot in chloroplast, were altered in cucumber cultivars.The above experiment results indicated that a positive relationship was observed...