Fungal Degradation Of Sea Buckthorn Proanthocyanidins Preliminary Study

Sea buckthorn proanthocyanidins is a kind of polyphenols with nutrition and health. The proanthocyanidins extracted from plants with low biological activity, it exists in form of polymers. The proanthocyanidins has strong physiological and pharmacological activity after the polymer be degraded into oligomer. Following the sea buckthorn falconoid and sea buckthorn oil, the development of Oligo-Sea buckthorn proanthocyanidins is the most important growth point of comprehensive utilization. There are two methods to degrade the proanthocyanidins polymer into oligomer, chemical degradation and biological degradation. The chemical degradation methods contain high-temperature hydrogenolysis and thiolysis etc. Exploring the methods of microbial degradation with safe and low cost will be high valuable for proanthocyanidins development and utilization.Through the earlier research works of laboratory, the microorganism that can degrade proanthocyanidins has been separated already. This thesis is identification of the fungi which can degrade proanthocyanidins, and using the fungi to the proanthocyanidins study.Using morphological and molecular biology methods to identify the fungi that can degrade proanthocyanidins, determined the fungi were Penicillium purpurogenum straj.Using the determined fungi to degrade proanthocyanidins, and using thin-layer chromatography to research the sea buckthorn proanthocyanidins before and after degradation. Then analyzed the change of oligomer proanthocyanidins content(OPC) before and after degradation, and the degradation effects of sea buckthorn proanthocyanidins were validated preliminarily. Using the macroporous resin column and SephadexLH20 to segregate and purify the sea buckthorn proanthocyanidins before and after degradation, then using Ultraviolet photometric detector to analyze the results of the separation and purification. The number of peaks appearing pre-degradation is less than the number of peaks appearing after-degradation. While the time of peaks appearing after-degradation is shorter than pre-degradation, it verified the degradation effects of fungi on Sea buckthorn proanthocyanidins. Purified samples were used in Free Radical Scavenging experiment, the pre-degradation scavenging rate of artificial free radical DPPH on sea buckthorn proanthocyanidins lower than after-degradation, and scavenging rate increased with the concentration.This study used Penicillium purpurogenum straj to degrade Sea buckthorn proanthocyanidins and obtain the oligomer. It has practical significance for the development and utilization of proanthocyanidins.