Clinical Research On The Effects Of Platelet Functions And Platelet Parameters In Patients Treated By Combination Chemotherapy Of Gemcitabine And Cisplatin

Objective To observe the changes of haemostatic function and morphology of the platelets before and after combination chemotherapy of gemcitabine (GEM) and cisplatin (DDP), to study the effect of chemotherapy medicines on the functions and morphology of platelet, to evaluate haemostatic function and bone marrow function of maligant tumor patients and provide theoretical basis and the experimental data to evaluate the safety of clinical chemotherapy medicine.Methods The blood is collected from fifteen selected patients before chemotherapy. The platelet aggregation function induced.by adenosine diphosphate (ADP) is measured by the turbidimetry, CD62P and PAC-1 are measured by the flow cytometry. Platelet count (PLT), platelet volume (MPV), platelet distribution width (PDW) as well as platelet large cell ratio (P-LCR) are measured by hematology analyzer. The same tests were performed in patients blood with thrombocytopenia fifteen days after gemcitabine and cisplatin combined with chemotherapy. Finally the changes of haemostatic function of the patients can be evaluated.Results The patients in accord with the selective standard results of the maximum platelet aggregation rates induced by ADP are 67.25±10.25% before chemotherapy and 64.61±10.05% after chemotherapy. The statistic analytic results with the paired-samples t test are not significant (p>0.05). The expression of platelet CD62P rates are 7.38±2.85% before chemotherapy and 7.89±3.51% after chemotherapy.The statistic analytic results with the paired-samples t test are not significant (p>0.05). The expression of platelet PAC-1 rates are 9.61±3.83% before chemotherapy and 9.91±3.74% after chemotherapy. The same statistic analysis can be drawn and the result is not significant (p>0.05). PLT, MPV, PDW and P-LCR are 207.93±46.62x109/L,11.16±0.84fl,13.58±2.26fl,38.41±4.12%before chemotherapy and 148.40±42.42×109/L,11.00±0.92 fl,14.19±2.09 fl,39.08±4.22% after chemotherapy. The statistic analytic results with the paired-samples t test are significant PLT, PDW (p<0.05) between prechemotherapy and post chemotherapy, MPV, P-LCR are not significant (p>0.05).Conclusion PDW increases significantly after gemcitabine and cisplatin combined chemotherapy, the results in the study indicate that the function and MPV, P-LCR of platelet remain unchanged.