| Objective: With the prevalence of coronary heart diseases and the popularity of coronary angiography, coronary angiography has become a commonly diagnostic tool to diagnose coronary heart diseases. Nevertheless, coronary angiography also is an invasive heart examination. Investigating all kinds of complications of this examination and giving some corresponding pretreatment might be important to reduce the complications to minimal degree. At present, the treatment of anticoagulation and antiplatelet aggregation had been standardized in procedure of percutanous coronary intervention. But the influence of coronary artery angiograph(CAG) on platelet function is seldom reported in literature. The anticoagulate therapy of CAG has not been given enough emphasis. In our clinical practice, we have found that there might be some new coronary artery occlusions when we performed PCI several days after CAG. Therefore, investigating the blood coagulation before and after CAG, searching for feasible standardized prevention and treatment measures for decreasing the incidence of acute coronary artery occlusion might have important clinical implications. Recently, manyresearchers reckon that platelet activation and thrombosis has close relation. Coronary heart diseases have close relation with thrombosis in occurrence, development, natural variation, treatment, prognoses judgement and so on. In other words, in every stages of CHD, (for example: atherosclerosis plaque formation, unstable angina, myocardium infarction, and so on.) all could appear hypercoagulability state that have the platelet high reactivity. Meanwhile, blood vessel endothelium function, blood coagulation and fibrinolytic system, hemorheologyall have a series of alteration. Among these, TXA2 released by platelets play an important role in coronary artery spasmus, myocardial ischemia, myocardial infarction and sudden cardiac death. At present, TXA2 existing in vivo is the strongest substance contracting blood vessel, and is the powerful inducer for platelet aggregation. Decreasing and inhibiting TXA2 generation has become one of the important methods to reduce the platelet aggregation. Aspirin could inhibit collagen, ADP, thrombin and antigen-antibody compounds, which could induce platelet aggregation, and aspirin could restrain platelet spontaneous aggregation. Platelet membrane protein GMP-140(CD62P, P-selection) and platelet membrane glycoproteins GPIIb/IHa which are the recipent compounds are specific markers of platelet activation .Flow cytometer can detect many parametes on single cell simultaneously. Therefore, it could examine CD62P GPII b/IIIa on the platelet membrane tojudge the state of platelet activation. This study observes the changes of CD62P and GPII b/IIIa before and after CAG, researches platelet activation caused by CAG and different influence caused by different contrast medium on platelet. At the same times, different dosages of aspirin were given to the patients 3 days before CAG and try to find out the best dosages of aspirin used as pretreatment.Methods: 54 patients were divided into 3 groups given on different dosage of aspirin respectively. Group l(n=18) with 75mg, group 2(n=18) with 150mg, group 3(n=18)with 300mg. Every group were subdivided to 2 subgroups, subgroup l(n=9) with Omnipaque(350), subgrroup 2 with Ultravist(370). Method of CAG: punctruring on right femoral artery by Judkins method. Coronary stricture was defined that: caliber stricture 50%. Every patient was examined with whole blood method by flow cytometer. Blood was drown to be test as preoperative sample after femoral puncturing and cannula inserting and before giving heparin, was drawn to be test as postoperative sample after cannula drawn out when CAG has been finished. All samples must be tested in 24 hours.Result: group 1 and group 2 have shown significantly difference in CD62P and GPIIb/IIIa between preoperative and postoperative (P < 0.05 ) .Group 3 have not shown significantly difference in CD62P and GPIIb/IIIa between preoperative and postoper...
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