Studies On Preparation And In Vitro Bioactivation Of Selenium Sulfated Lycium Barbarum Polysaccharides

Polysaccharides are a class of in vivo non-specific immune-enhancing compounds. Lycium barbarum polysaccharides (LBP) are the main bioactivation constituent with in Lycium barbarum. The chemically modification of LBP has important academic sense and applied value for the elevation of their bioactivations and the exploitation of new drugs. In this work sulfated lycium barbarum polysaccharides and selenium sulfated lycium barbarum polysaccharides were prepared bychemically modification, and their bioactivations were investigated in vitro. This paper includes the following aspects:Chapter1:The research progress for preparation and bioactivation of sulfated polysaccharide and selenium polysaccharides was reviewed.Chapter2:Lycium barbarum polysaccharide was sulfated by using chlorsulfonic acid-pyridine method. The modification conditions were optimized by Lg(34) orthogonal design. The degree of substitution (DS) was tested by sulfate barium-turbidimetric method. The nature of Sulfated polysaccharide of LBP was characterized by UV spectra and Fourier transform infrared spectra analysis. The inhibiting effect of SLBP to growth of Hela cell in Vitro was tested with MTT method. At a concentration of 200μg·mL-1, LBP sulfates on Hela cell inhibition rate of 31.71%.Chapter3:The self-made LBP sulfate was modified with selenium. UV and IR spectra of LBP sulfate and selenium-modified products were surveyed. The inhibiting effect of LBP sulfate and selenium-modified products on human cervical cancer Hela cell was investigated by using MTT method, and their antioxidant activity was also evaluated by spectrophotometry. The results showed that the inhibition rate of selenium sulfate LBP on the growth of human cervical cancer Hela cell increased significantly, comparing with sulfate LBP. The inhibiting rate of selenium sulfate LBP on Hela cell was 59.67%, and its hydroxyl radical scavenging rate was 60.51% for selenium sulfate LBP and 38.9% for LBP sulfate. A better superoxide radical scavenging rate was also achieved.