| Background and objective:With the development of society, various of electronic equipments are used in our daily life and work, which leads to more and more serious radiation pollution. At the same time,due to more and more use in clinical treatment,radiation damages are paid increasing attention.Nowadays,with the deterioration of environment pollution and food safety,etc,the rates of tumor increase.Radiotherapy is still one of the important treatments of malignant tumor,and especially when irradiating the lower abdominal it can damage male reproductive system,so radiation has a great effect on male reproductive system. Androgen binding protein is synthesized and secreted by Sertoli cells,which can combine with androgen to keep up the level of androgen in spermatogenic tubule so that it can formate high concentration androgen environment to promote spermatogenesis. At present there are few reports about the effect of radiation on ABP, and fewer about the drug which can protect the ABP damage caused by radiation, so researchers pay more and more attention to seeking a protective agent of less side effect.Chinese herbal medicine is our country’s traditional drug,Many Chinese herbs have anti-radiation effect, but Chinese medicine ingredients are complex and clinical repeatability is poor, so we should choose the effective components, and carry on qualitative and quantitative research. The active components of Lycium barbary polysaccharide(LBP) had a protective effect on radiation of the damage. It’s protection is associated with the antioxidation ability, which can increase the weight of rat reproductive organs and can have certain effects on serum sex hormone’s level.This article aims to investigate the effect of radiation on ABP, and whether LBP has recovering effects on ABP,which can provide theoretical support for promoting polysaccharides’use in clinical treatment. Methods:Forty-five male rats were randomly divided into3groups:the normal group(A),the irradiating model group(B),the LBP group(C).Except A group,other groups were given radiations with a dose of6Gy60Co-γ ray twice,once a week.24hours after modelling,Rats of C group were given LBP polysaccharades(80m g/(kg.d)).Rats of A and B group were given equal volume of physiological saline once a day for two weeks. The morphology of the testicular tissues were observed. The levels of serum follicle-stimulating hormone (FSH), luteinizing hormone (LH),and testosterone (T)were determined by ELISA. The relative expressions of ABP mRNA and GSH-PxmRNA in the testicular tissues were determined by PCR.Results:The spermatogenic epithelium in normal group was complete,in which we could see sertoli cells,spermatogonia,spermatocytes of different phages,spermatids and sperm.They were arranged orderly and distinctly.There were vacuoles in model group’s spermatogenic cells and sertoli cells,in whose seminiferous tublue we could see fewer sertoli cells,scarce spermatocytes.The LBP group’s spermatogenic function and sertoli cells were improved significantly.As for sex hormone,the levels of FSH and T increased greatly,LH changed a little,the levels of ABP and GSH-Px in testis increased.The model grorp’s levels of serum T and FSH changed greatly,and the levels of ABP and GSH-Px decreased significantly,whose differences were statistically significant. Compared with the model group,the levels of FSH and T,the expression of ABP mRNA and GSH-PxmRNA in testicular tissue of LBP group were improved greatly(P<0.05).Conclusion:Radiation has obvious inhibitory effects on ABP, and LBP can improve the expression of ABP mRNA in rat’s testis damaged by60Co-γ. LBP protection effects are associated with the antioxidant effects and the improvements of T level. |
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