| Objective To investigate the role of lycium barbarum polysaccharide(LBP)in effect of mouse titanium particles on inducing joint in skull osteolysis model,and to investigete the mechanism of lycium barbarum polysaccharide in aseptic loosening.Methods In the experiment,the mouse skull osteolysis model was used to investigate the effect of lycium barbarum polysaccharide on osteolysis in mice.The effect of LBP and lycium barbarum polysaccharide on the effect of LBP in the sham operation group,positive control group,low and high dose groups was also investigated.The rats in the control group were given gavage 20 mg /(kg · d)or 100 mg /(kg · d)once a week later,while the control group were given the same volume of saline for gavage.In the positive control group,the titanium particles were implanted into the skull Did not give any drugs,2 weeks after gavage sacrificed.The thickness,number of trabecular bone and the surrounding osteolysis of prosthesis were observed by Micro-CT.Western blot was used to measure RANKL and OPG levels.The expression of RANKL and OPG in the joint tissues was observed by immunohistochemical staining.The levels of IL-1,TNF-?,OPG and RANKL were measured by ELISA.Results Micro-CT scans of the wild-type male C57 skull model clearly show the presence of osseous bone around the skull-bearing titanium particles in the positive control group,but at low drug doses and high The extent and extent of bone resorption in the drug group were significantly reduced,while there was no obvious bone resorptionand destruction in the sham group.HE staining results,the control group and the low drug volume group,high drug volume group results compared to the control group can be found around the skull bone mass was significantly less than taking LBP two other drugs group,low drug volume group.The bone mass decreased more significantly than the high drug group.In the same position as the immunohistochemical staining results,there were similar gradient distribution results in the number of OPG staining and positive staining cells from the high drug volume to the low drug volume to the control group followed by reduced;the trend of RANKL staining is the opposite.The results of IL-1?and TNF-? showed that no obvious inflammatory factors were found in the blank control group,while the inflammatory factors around the skull tissue of the mice in the positive control group were the most,and the high concentration of sputum polysaccharide in the surrounding tissues of the skull after intragastric administration.Significant reduction in inflammatory factors.The positive control mice The tissue around the skull contains the most inflammatory factors.The expression of OPG and RANKL in the samples of four groups of experimental mice was determined by Western-blot.The expression of RANKL protein was highest in the positive control group,and the protein expression decreased with the increase of the concentration of lycium polysaccharide.The expression of OPG protein was opposite to that of RANKL protein.The content of OPG protein in the polysaccharide-administered group was higher than that in the positive control group,and the higher the concentration of lycium polysaccharide,the more the expression of OPG protein.Conclusion Lycium barbarum polysaccharides can inhibit the expression of inflammation-related factors,and inhibit the bone-induced osteolysis in mouse skull.Lycium barbarum polysaccharides can inhibit the osteolysis-induced osteolysis in mouse skull,which may influence the aseptic loosening caused by worn particles after jointreplacement by up-regulating the ratio of OPG/RANKL and inhibiting the production of inflammatory factors. |
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