Naotaifang Atherosclerotic Rabbit Oxidative Stress

Objective:To research Naotaifang for the role of oxidative stress in the rabbit model of atherosclerosis by preying and its mechanism.Methods:96 Healthy Japanese white rabbits were male and female in half, were randomly divided into two groups:control group (n=10) and model group (n=86). The establishment of a rabbit model of atherosclerosis by using immune injury and feeding high fat diet plus, By detecting the blood lipids and vascular pathological biopsy to evaluate model successful established.Successful model animals would been completely randomly divided into six layers, such as the control group,model group, Atorvastatin group, Jiangzhining group, low-dose group of Naotaifang, middle-dose group of Naotaifang, high-dose group of Naotaifang. Animals were fed treatment one month. By measuring blood lipids, serum SOD,MDA, ox-LDL and brain tissue SOD, MDA levels; Viewing vascular intimal lesions and plaque distribution by naked-eye,Observing the pathological change of the vascular and Measuring plaque thickness and plaque area under light microscope;Measuring the expression of LOX-1, MCP-1, ICAM-1, VCAM-1 mRNA By RT-PCR and the protein expression of LOX-1, MCP-1, ICAM-1, VCAM-1 by Western-Blot. Using RT-PCR and Western-blot methods to determinate the mRNA and protein expression levels of LOX-1, MCP-1, ICAM-1, VCAM-1 in the rabbit aorta. Results:90 days, the serum TC, LDL-C, MDA, ox-LDL of treatment groups of Naotaifang compared with the model group were significantly lower, the serum SOD was significantly higher (P<0.05, P<0.01).The middle and high dose group of Naotaifang compared with the positive control group of Traditional Chinese medicine and Western medicine have no significant difference, Naotaifang can reduce blood lipids, increase SOD enzyme activity and decrease production of MDA, inhibit LDL oxidation, reduce ox-LDL generation.Light microscope:the treatment group of Naotaifang aortic pathological changes were lighter than the model group, and compared with the positive control groups, there is no significant difference. Results of RT-PCR detection showed that the control group basically have no LOX-1, MCP-1, ICAM-1, VCAM-1mRNA expression, and the expression of model group was significantly higher than normal (P<0.05), and The expression of the treatment groups than the atherosclerosis model group were significantly lower (P<0.05), Naotaifang treatment groups have no significant difference with positive control groups.Wertern-blot determination results show:The model group LOX-1, MCP-1, ICAM-1, VCAM-1 protein expression was significantly higher than the control group, the treatment groups compared with the model group,the protein expression of LOX-1, MCP-1, ICAM-1, VCAM-1 were decreased (P<0.05),the middle dose group of Naotaifang compared with the positive control group,there are no significant difference. Naotaifang can reduce the expression of inflammatory mediators through antioxidant mechanism, By inhibiting the role of ox-LDL that can induced LOX-1 expression in endothelial cells and induce endothelial cells to produce adhesion molecules, which reduced the expression of LOX-1, MCP-1, ICAM-1, VCAM-1, reduce the uptake of endothelial cells, smooth musclecells, macrophages on ox-LDL, thus inhibiting the formation of foam cells, thus protect endothelial function and inhibit the occurrence and development of AS.Conclusion:Naotaifang has many interactions by reducing blood lipids, oxidativing stress, reducing inflammation and protecting vascular endothelial function. Inhibiting the LDL oxidation, reducing the formation of ox-LDL, reduced vascular endothelial LOX-1, MCP-1, ICAM-1, VCAM-1mRNA expression, which may be one of the mechanisms of the anti-atherosclerosis oxidative stress.