Salvia Combined With Bone Marrow Mesenchymal Stem Cell Transplantation On Angiogenesis Of The Infarcted Myocardium

An experimental study of Danshen intervention to Bone marrow-derived mesenchymal stem cells transplantation in the treatment of acute miocardial infarction about Angiogenesis.Purpose:To evaluate the SM intervention mesenchymal stem cells in zero-day, seven days of intravenous injection on rabbits with acute myocardial infarction infarction angiogenesis.Material and method:70 randomly divided into normal group, 10 rats in model 60(according to pre-experimental modeling minimum 70% success rate). MI model successfully reproduced in the model were randomly divided into model, zero-day injection of stem cell group, zero-day intravenous Danshen injection(SM) + marrow MSCs group and the seventh day intravenous SM + marrow MSCs group. N = 10 in each group no significant difference in body weight(P> 0.05).(1)normal group: no intervention,(2)model group: simple modeling,(3)intravenous injection of zero-day stem cells Group: After the suspension immediately to the stem cells injected with 2×10~6 cells,(4)Zero-day intravenous SM + MSCs group: after the formation of acute myocardial infarction, immediately to the stem cell suspension 2×10~6 cells single intravenous injection, while I prepared by 15.4mg/kg Danshen injection, on an intravenous drip for 10 days,(5)seven days intravenous injection of MSCs + SM group: After the preparation of Salvia to 15.4mg/kg injection on an intravenous drip for 10 days, the seventh day of intravenous injection of Danshen and bone marrow MSCs groups: acute formed 1 week after myocardial infarction, to the stem cell suspension 2×10~6 cells single intravenous injection.Results:CD31 immunohistochemical analysis of integrated optical density values:Compared with normal group were lower in other groups, in addition to zero Heavenly Stems Cells + SM group the other group were statistically significant(P <0.05), compared with model group, stem cell group, zero Heavenly Stems cells + SM group, seven days a stem cell + SM group had higher(P <0.05), stem cell group, zero Heavenly Stems cells + SM group, seven days a stem cell + SM group was no significant difference between groups. bFGF, VEGF was no significant difference among the groups.Conclusion:1.Point transplantation of bone marrow mesenchymal stem cells, can be moved totheinfarctzone.2.Bone marrow mesenchymal stem cell transplantation for myocardial infarction and surrounding area infarction angiogenesis.3.Danshen can enhance bone marrow mesenchymal stem cells promote angiogenesis, but there was no difference.4.In the zero-day and seven days of two time points stem cell transplantation combined with Danshen injection were no different.