The Yifei Drink 549 </ Sub> Human Lung Cancer Cell Lines A, <sub> Experimental Study

The latest research shows that the occurrence of cancer is the consequence of long-term interaction of polyactors and polyphases the definition of apoptosis is constructed by kerr, an Australian pathologist, in the year 1972. It is an initiatively programmed cell death (PCD) procedure, which is a universal mechanism in multicelluar organism. PCD plays an important role in maintaining the relatively stability of the numbers of cells. Once it is interrupted, the tumor or other diseases will probably come into being. The procedure of the death of cells has been proved to be closely concerned with the occurrence of cancer. The balance of the tissue is the result of balance of the proliferation and death of cells, the overgrowth is because of the death rate of cell is too low while the growth rate is too high, if the growth of cancer tissue in under control or even the PCD is functioning. It cancer cells will stop growing, and the normal state of the body will be maintained.Until now the principal therapy of lung cancer including surgery, radiotherapy and chemical therapy will caused severe side effects. The traditional Chinese medicine has shown its own advantages in clinical practice. Yi-Fei-Yin is an effective medicine in the treatment of lung cancer. Using the serum contained Chinese herbs 3d-2t-2h,we think it is the reliable serum pharmacology method to research the action of Yi-Fei-Yin to the human lung cell A549.After building the tumor animal model successfully, we divided the animal into three groups: Saline control group; cisplatin chemiotherapy group; Yi-Fei-Yin treating group. Using the method of light microscope (LM) and electron microscope (EM) to observe the morphology. And enzyme cytochemistry was used to detect the activity of G-6-Pase and Mg2+-ATPase of the lung cancer cell. Through the observation of EM, we found the formation of apoptotic body in the Chinese herbs group. It is the most characteristic change of the apoptosis cell. Enzyme cytochemistry method showed that the response granules of G-6-Pase and Mg2+-ATPase in the human lung cell A549 become smaller after the treatment of serum contained Chinese herbs, the amount of the granules became fewer, the density was lower, some were negative. The activity of the enzyme decreased obviously.Conclusion: 1. Yi-Fei-Yin .The inhibitory function of serum-contained Yi-Fei-Yin to the lung cancer reacts through multipath and multiple factor. Its important mechanism of resisting cancer is inducing the apoptosis of tumor cell. 2. Inhibiting the activity of the telomeraseA549 and G-6-Pase and Mg2+-ATPase.