The Experimental Research Of The Effects Of Wei-Ai-Kang To Human Stomach Cell Line SGC-7901

Purpose: To study the effects and mechanism of WEI-AI-KANG on the stomach cancer cells and to provide reliable evidence to future application to clinical practice.Methods: In vitro, the human stomach cancer cell line SGC-7901 were cultured. The alteration of proliferation and activity of enzymes of cancer cells was observed by applying the enzyme cytochemical method, and the apoptosis was determined by light microscopy, electron microscopy.Results: (1) The serum containing Chinese herbs has an obvious inhibitory function on the human stomach cancer cell line SGC-7901, and WEI-AI-KANG showed time-dependence inhibitory effects. The inhibiting rate is 48.63% after 24hs' culture, 63.18% after 48hs' culture. (2) The observation of LM shows the morphological change of the stomach cells of Chinese herbs group. (3)The observation of EM shows the formation of the apoptotic cell. (4) Enzyme cytochemical method indicates that the response granules of G-6-Pase and Mg²⁺-ATPase in the colon cell become smaller after the treatment of serum contained Chinese herbs, the amount of the granules become fewer, the density is lower, some are negative. The activity of the enzyme decreased obviously.Conclusions: (1) WEI-AI-KANG has the function of inhibiting the proliferation of the human stomach cancer cell line SGC-7901. (2) WEI-AI-KANG can decrease the activity of the G-6-Pase and Mg²⁺-ATPase of the human stomach cancer cell line SGC-7901 has the function of inhibiting the proliferation of the human stomach cancer cell line SGC-7901. (3) WEI-AI-KANG can induce the cancer cell apoptosis. (4) WEI-AI-KANG can inhibit human stomach cancer cells' ability of invasion and metastasis.