Colorectal Cancer Rather Ccl229 Human Colon Cancer Cell Lines The Role Of Experimental Research

Purpose: To study the effects of CHANG-AI-NING on the proliferation and activity of enzymes of colon cancer cells and explain the mechanism of its anti-tumor function.Methods: In vitro, the human colon cancer cell line CCL229 were cultured. Growth curve was evaluated by MTT assay , the activity of G-6-Pase and Mg²⁺-ATPase was detected by applying the enzyme cytochemical method, and the apoptosis was determined by light microscopy, electron microscopy and agarose gel electrophoresis.Results: (1) The serum containing Chinese herbs has an obvious inhibitory function on the human colon cancer cells CCL229, and CHANG-AI-NING showed time-dependence inhibitory effects. The inhibiting rate is 53.85% after 24hs' culture, 63.49% after 48hs' culture. (2) The observation of LM shows the morphological change of the colon cells of Chinese herbs group. (3) The observation of EM shows the formation of the apoptotic body, which is the most characteristic change of the apoptosis cells. (4) The observation of EM also shows the decreased amount of the microvilli, which play a key role in the process of invasion and metastasis. (5) Enzyme cytochemical method indicates that the response granules of G-6-Pase and Mg²⁺-ATPase in the colon cell become smaller after the treatment of serum contained Chinese herbs, the amount of the granules become fewer, the density is lower, some are negative. The activity of the enzyme decreased obviously.Conclusions: (1) CHANG-AI-NING has the function of inhibiting the proliferation of the human colon cancer cell CCL229. (2) CHANG-AI-NING can induce the human colon cancer cell apoptosis. (3) CHANG-AI-NING can decrease the activity of the G-6-Pase and Mg²⁺-ATPase of the human colon cancer cell. (4) CHANG-AI-NING can inhibit human colon cancer cells' ability of invasion and metastasis.