Panax Extracts Inhibit The Malignant Transformation Of Human Gastric Epithelial Cells, The Role Of Research

Gastric cancer, the most-commonly seen in China, is one of the malignant cancers with the highest incidence and mortality. Because the first-grade prevention targeted at pathogeny is relatively difficult, it's important to strenghen the research and prevention of precancerous lesions.At present, Western medicine shows little effect in reversing precancerous lesions .There are many clinical studies about the mechanism of Traditional Chinese Medicine(TCM) in treating gastric precancerous lesions. Most of these studies are about compound prescriptions and there is few reports about the study of simple prescriptions. In order to solve this problem, we studied the inhibiting effects on precancerous gastric cells by the serum of the dog being fed with distilled substances of SanQi(penex notogingseng) through the method of cells culture and tried to find the points or periods of time when the SanQi's function was the strongest.The mechanism of promoting apoptosis was further investigated, providing some theoretic foundation for developing new medicine.l.The inhibiting effects of the dog serum on the proliferation of GES-1 and MC cellsThe experiments adopted eternalized human gastric mucosa epithelium GES-1 cells and MC cells gained from GES-1 cells transformed by MNNT as the model of precancerous lesions for study in vitro. Feeding the dog with SanQi distilled substances one time, we took the serum befor feeding, and 5, 10, 30, 60, 120, 180, 240, 300, 360, 480, 600, 720, 840, 960, 1200, 1440 minites afer feeding as the medicated serum after destroying alexins.We examined the inhibiting effects 72 hours after function on GES-1 and MC cells of different concentration of medicated serum(8 % * 4 % , 2% )gained from different points of time by means of MTT, so as to find the best functional points of time.The results show that GES-1 and MC cells inhibition rate is the highest at 2 hour and 6 hour- medicated serum.At these two points, the culture medium containing 8% medicated serum has prominent inhibiting effects and quantity-effect relation are observed at each point. In order to further discuss the mechanism of inhibiting effect of medicated serum on proliferation of precancerous gastric cells, the following experiments are implemented. 2. The apoptosis-promoting effects of the dog serum on MC cellsAt these two points of time, different concentration of medicated serums were used to promote the apoptosis. Results showed that the serums has significant effects of promoting apoptosis at these two points of time.Compared with non-medicated serums under same conditions, the proportion of apoptosis had a significant increase(p<0.05. MC cells' proportion was decreased in GO/Gl period (p<0.05 ) and increased in the G2/M period ( p<0.05 ) ,while no consistant changes are observed in S period.To summarize, the results of the study on the cell models in vitro of gastric precancerous lesions showed that the points of time with the strongest inhibiting effects is 2 hours and 6 hours after being fed with the SanQi substance.At these two points, the serums had significant effects of promoting apoptosis, Dramatically decreasing MC cells' proportion in GO/Gl period and increasing it in the G2/M period were probably responsible for the effects of inhibiting the proliferation of MC cells and promoting apoptosis.