Ctla4-1gg Study On The Role In Pancreas Transplantation In Rats With Diabetes

Objective : To evaluate the effect of CTLA4-IgG on the immunological tolerance of pancreaticoduodenal transplantation model in allogenic rats.Methods: The diabetes Wistar rats as recipients were induced by intravenous administration streptozotocin (STZ) in a single dose of 60mg/ kg of body weight via penis dorsal vein. The diabetes rats were randomly assigned to control group (n = 16) and transplantation group (n=16). Pancreaticoduodenal transplantations were done with the SD rats as donors,then the graft including duodenum and pancreas were harvested enbloc, while the abdominal aorta segment including the coeliac artery and mesentery artery were kept intact. In the recipient operations, the left kidneys was removed while the right kidney was kept intact, the recipient left renal vein was anastomosed with the donor portal vein by end-to-end fashion, the recipient abdominal aorta was anastomosed with the donor proximal end of donor abdominal aorta by lateral-to-end fashion, and the recipient small intestine was anastomosed with the donor duodenum by lateral-to-lateral fashion. The operation group were administered via celiac injection with CTLA4-IgG during operation and after operation(from the 1st day to 7th day) The control group were administered via celiac injection with axenic citric acid puffer solution in the same way as the operation group .Blood glucose of the two recipient groups were investigated daily after transplantation for 7 days, The graft functional survive interval (GFSI) was recorded synchronously. The concentration of IL-2 was observed with ELISA on the 3rd, 7th day after operation. Pancreas tissue biopsies were done on the 3rd,...