| Comparative Research on HPLC fingerprint of Cordyceps sinensis and its cultured mycelia, and to study on the chemical constituents from the Cordyceps sinensis Mycelium, the mainly solutes are as follows:1 To study the cordyceps sinesis and its cultured mycelia by HPLC and TLC, There were 6 common peaks in HPLC fingerprint of Cordyceps corensis and its cultured mycelia ,three of which were determined as Uracil, Adenosine, Cordycepin.2 12 compounds were isolated from Cordyceps sinensis Mycelium .By means of both solvents extraction and phasechromatography , on the basis of physico-chemical properties and spectral data , 12 compounds were identified as follows:β-sitosterol (1), stigmasterol (2), ergosterol (3), ergosterol peroxide (4), 7 (8) ,22-dien-3,5,6-triol-ergostane (5), n-pentacosanoic acid (6), methylp hydroxybenzeneacetate (7), 3,5-dimethoxy-4-hydroxybenzoic acid (8), genistein (9), gensitin (10), daidzein (11), adenosine (12) Compound 6, 7, 8, 9, 10, 11 were isolated from this genus for the first time.4 Comparative Research on HPLC fingerprint of Cordyceps sinensis and its cultured mycelia, and established the extraction method and HPLC method to analyze the Cordyceps sinensis and its cultured mycelia in our study on fingerprints of cordyceps. |
