Chek Huang Pingyang Soup, Pairs Of Female Rats With Anti-sperm Antibody Inhibition Experiments

ObjectiveIn order to clarify that "duxie" is the basic pathogenesis of Anti-sperm Antibody, thispaper estabilished anti-sperm antibody masculine animal models with the female rats, andused by active immunization of homologous modified character method,studied the effectsof Chihuangpingyangtang on OD of anti-sperm antibody, pregnancy rate,the number ofembryo,the weight of organ and observed pathologic changes of uterus, ovary to provedrestraint role of Chihuangpingyangtang and offered gist for clinical medicine.MethodsTotal 50 female rats at the age of 2 to 3 months,ten rats were randomly selected asnormal control group,another 40 rats were separately injected antigen to get immunearound subcutaneous lymph nodes of bilateral inguinal. After immunizations for fivetimes,all of female rats'serum was positive and these rats were randomly divided intomodel group by random number table method, Prednisone group, Phihuanpingyangtanggroup, Zhibaidihuangtang group,which were separately given oral medicine.Normalgroup and model group were administered with physiologic saline orally. After two weeks,blood was adopted from eyes,separated serum and detected anti-sperm antibody of serumby enzyme linked immune sorbet assay. At the same time,to collect group statistics of thenumber of embryos and pregnancy rate after 18 days when 3 male rats were added to thefemale in each group. To explore the histology changes of rats'ovaries and uteruses andrecord the weight of liver, spleen,kidney when dissected rats.Results1.The effect Of AsAb OD on medicine:after immunizations for five times,fortyfemale rats'serum was positive and there is no difference about AsAb OD.animal modelswas estabilished successfully.Before mating,AsAb OD of Chihuanpingyangtang andPrednisone group was lower than model group.After about twenty days, AsAb OD ofeach group were more lower than model group, Chihuangpingyangtang group had bettereffect than Zhibaidihuangtang group and prednisone group.2.The number of embryo and pregnancy rate:The pregnancy, rate of Chihuanpingyangtang,zhibaidihuangtang and prednisone group is higher than modelgroup. There is no statistics because of specimen. The number of embryo of model groupare lower than the normal group. The number of embryo Chihuangpingyangtang groupmuch more than model group.3.The weight of organ:The weight of spleen of model and prednisone group is lowerthan normal group.there are no difference each group about the weight of liver and kidney.4.There is no obvious change of uterus and ovary.ConclusionsChihuangpingyangtang could decrease AsAb OD and increase the number ofembryo while the weight of organ don't be drop.