Resistance To Fatigue Parties Fatigue Serum Testosterone, Interleukin-6 Content And Apoptosis Of Lymphocytes In The Impact Study

ObjectiveTo find out the basic Traditional Chinese Medicine pathologensis of chronicfatigue syndrome, and create chronic fatigue syndrome animal model.To study themechanism of chronic fatigue syndrome in lymphocyte apoptosis and further clarifythe mechanism of anti-fatigue prescription therapying chronic fatigue syndrome.Method1.Wistar male rats were divided into 3 groups:normal group,model group, anti-fatigue prescription group.Using compound agents to make chronic fatigue animalmodel:rats accepted 14 days compound agents' stimulation,that is to say,on odd-numbered days swims in cold water for 30 minutes, on even-numbered days matedwith rut female rats; Every day clips the rat's tail to stimulate for 3 hours.2.In open field, strength exhaust,swim and rat's tail suspending to observe thechange of praxiology.3.Using radio-immunity to detect the content of Interleukin-6 in blood serum.4.Detecting the content of Testosterone and Cortisol in plasm using radio-immunity.5.Detecting the change of lymphocyte apoptosis rate in the peripheral bloodusing Flow cytometry.6.Using Flow cytometry to detect the expression of bcl-2 in the peripheral blood.Result1.The model group has phanero-anomaly changes: body weight descent, hairabscission, lazy, stool no- shape, appetite descent,and easy to rage,et al.2.The content of Interleukin-6 in blood serum was lower than the nomal, anti-fatigue prescription group's change significant relieve.3.The content of Cortisol in plasm was higher than the nomal, Testosterone'scontent was lower, T/C decrease signifcantly and anti-fatigue prescription group'ssignificantly increase.4.The lymphocyte apoptosis rate in the peripheral blood was higher than thenomal, anti- fatigue prescription group's near to the nomal.5.The expression of bcl-2 in the peripheral blood was lower than the nomal, anti-fatigue prescription group has no difference with the nomal. ConclusionWe succeeded in creating the chronic fatigue syndrome animal model,andsuggested standpoint that "excess of liver, deficiency of Spleen and Kidney" is thebasic Traditional Chinese Medicine pathologensis of chronic fatigue syndrome.Itsuggested that lymphocyte apoptosis is related with chronic fatigue syndrome ,and wefound that model rats' behavior changed, the content of Interleukin-6, Testosterone inblood serum was low, meanwhile Cortisol was high, The lymphocyte apoptosis ratewas higher and the expression of bcl-2 in the peripheral blood was lower than thenomal, it makes clear that the model rat was fatigue, various kinds of compoundstress result in correlated biochemistry factors' disorder was the main mechanism.Thestudy manifest that anti- fatigue prescription can adjust and recovery the factors,wesuspect it can regulate the nerve-endocrine-immunological network.The anti- fatigue prescription's effect disproof that chronic fatigue syndromeTraditional Chinese Medicine pathologensis is reasonable and scientific.