Picrorhiza Study On The Chemical Constituents Of Tibet

The plant Picrorhiza scrophulariiflora Pennell(Scrophulariaceae)grows in high-altitude regions(above 4400 m)in southeast Tibet and northwest Yunnan province in China.The root of this plant is used in traditional Chinese medicine for the treatment of damp-heat dysentery,jaundice,indigestion,common fever,acute viral hepatitis,and steaming of bone.For the purpose of clear the material base for its bioactivity and provides the theory basis for the successor development research on the root of Picrorhiza scrophulariiflora Pennell,the chemical constituents investigation on the acetic ether layer and n-butanol layer of the 95%ethanol extracts were carried out.By using silica gel C.C.,Sephadex LH-20 and ODS, 34 compounds were isolated,morever,the structures of 28 compounds were identified by the physical-chemical properties and spectral methods,which were identified as hexacosanol(1),β-sitosterol(2),daucosterol(3),25-acetoxy-2-β-D-glucopyranosyloxy-3,16,20-trihydroxy-9-methyl-19-norlanosta-5, 23-diene-22-one(4),2-β-D-glucopyranosyloxy-3,16,20,25-tetrahydroxy-9-methyl-19-norlanosta-5, 23-diene-22-one(5),vanillic acid(6),gallic acid(7), arbutin(8),androsin(9),scroside G(10),scrophenoside A(11),catechin(12),luteolin(13), luteolin-7-O-β-D-glucopyranoside(14),11-O-(4'-O-methylgalloyl)-bergenin(15),ll-O-galloylbergenin (16),bergenin(17),minecoside(18),picrosideⅠ(19),picrosideⅡ(20), sweroside(21),gentiopicroside(22),picrosideⅤ(23),isoferulic acid(24),caffeic acid-4-O-β-D-glucopyranoside(25),methyl caffeate-4-O-β-D-glucopyranoside(26),1, 6-dicaffeoyl-O-β-D-glucopyranoside(27),scrocaffeside A(28).Among them compound 1,7, 8,12-17,21,22,24-27 were isolated from Picrorhiza genus for the first time,compounds 23 and 28 were novel compounds.