Invigorating Kidney Of Tgf-beta <sub> 2 </ Sub> State Intervention In High-sugar Retinal M¨¹ller Cell Glutamate Uptake Of Research

Objective : To observe the change of vitro retinal Müller cells with the highglucose level and transforming growth factor-β₂(TGF-β₂) intervention, and to explore the mechanism of action of the therapy called Bu-Shen-Huo-Xue(BSHX) primarily. Further more,to provide the experimental evidence of therapy BSHX used in treating the diabetic retinopathy (DRP).Methods: 1. Modified enzyme-digesting was employed in culturing retinal Müllercells of Newborn-rats. 2. The ultrastructure of retinal Müller cells was observed by transmission electron microscope(TEM) .3. The glutamine synthetase (GS) activity and the lactate dehydrogenase (LDH) leakage of retinal Müller cells was detected by Spectrophotometer. 4. The glutamate (Glu) intakes of retinal Müller cells was measured by the liquid scintillation detector.Results: 1. In the high glucose level condition, the ultrastructures of retinal Müllercells were damaged obviously. 2. During the 12-hour period, the retinal Müller cells GS activity can be significantly improved by BSHX medicine-containing serum in the normal condition ,and can be significantly improved by BSHX medicine-containing serum with the interventions of the TGF-β₂ and high glucose level during the 12-hour and 24-hour periods. 3. During the 24-hour period, the vigor of retinal Müller cells can be significantly improved by BSHX medicine-containing serum both in the normal condition and the condition with interventions of the TGF-β₂ and high glucose level.4. During the 24-hour period, the condition of retinal Müller cells' abilities to absorb Glu which can be significantly improved by BSHX medicine-containing serum in normal condition, but in the condition of both TGF-β₂ and high glucose level cannot.Conclusion: 1. The ultrastructures of retinal Müller cell can be damaged obviously in high glucose level condition, which may be the basis of functional changes in the DRP.2. BSHX medicine-containing serum can improve the GS activity of retinal Müller cell, and stabilize cells' membrane, degrade its permeability, enhance cells...