Mechanism Of Ruanjian Gall Soup Prevention Of Chronic Lymphocytic Thyroiditis

Purpose: To evaluate the effects of the traditional complex prescription Ruanjianxiaoying decoction in treatment of the EAT mice through the TGAb,TPOAb,IL-1,6,TNF-α,the pathomorphology and apoptosis-regulated proteins Fas/Fasl,Bcl-2/Bax change of the thyroid gland . Investigate the effect Mechanism from multiple target point.Material and method:An animal model of experimental autoimmune thyroiditis ( EAT) in rats was developed by injecting Tg and CFA two times,and the interval was two days. Then inject the mice with Tg and IFA for the next three weeks as strengthening the immune.Meanwhile,feed the EAT mice with high iodine water(1 liter water add to 0.64g NaI). So the model was completed . Take the experimental group serum to detect level of thyroglobulin antibody and then all the mice were divided into four groups randomly. They were normal group, Ruanjianxiaoying decoction treatment group , Glucosidorum Tripterygll Totorum treatment group and EAT group.Treat the mice for six weeks with Ruanjianxiaoying decoction:(1)Detect the levels of autoantibodies with Enzyme-linked immunosorbent assay.(2)Observe the level change of Fas/FasL,Bcl-2/Bax in the thyroid of rat with Immunohistochemical staining.(3)Survey the pathomorphology change of the thyroid gland under the light microscope(4)Compare the results between every groups.Results:1. Comparing the Ruanjianxiaoying decoction treatment group with the EAT group: the level of the TgAb,TpoAb,IL-1,6,TNF-α,Fas/Fasl and Bax were significantly lower.and the level of the Bcl-2 were higher. The pathomorphology change of the thyroid gland were better than that of the EAT group.2. Comparing the Glucosidorum Tripterygll Totorum treatment group with the EAT group: the level of the TgAb,TpoAb,IL-1,6,TNF-α,Fas/Fasl and Bax were more lower.and the level of the Bcl-2 were higher. The pathomorphology change of the thyroid gland were better than that of the EAT group.3. Comparing the Ruanjianxiaoying decoction treatment group with the Glucosidorum Tripterygll Totorum treatment group: the efficacy of the Ruanjianxiaoying decoction treatment group was better.Conclusion:1. Ruanjian Xiaoying Decoction has the function of lowering the expressions of antibodies and cytokines in serum levels of the EAT mice.2. Ruanjianxiaoying Decoction has the function of reducing the apoptosis of the thyroid of the EAT mice.3. Ruanjian Xiaoying Decoction has the function of alleviating the pathological injury of the thyroid of the EAT mice.