Curculigo Toxicity Test Of Extracts

1,Research purposesWu Shu Technique with increasing difficulty, in Martial Arts due to the increasing risk of various injuries, taking traditional Chinese medicine has become the sport of Wushu athletes to raise the level of an effective way to prevent sports injuries. Medicine Curculigo their physical health, enhance immunity, kidney impotence and other functions, one is Chinese Wushu uniforms, the current research in Chinese medicine Curculigo concentrated in active ingredients, pharmacology, efficacy, etc,of their toxic Side research, such as toxic dose, the targets of toxicity, the mechanism of toxicity and prevention of emergency means a lack of systematic study areas.In this paper, the acute toxicity test in rats, rat poisoning and determination of the water extract of Curculigo taking the maximum tolerated dose and related parameters, suggesting that the target organ toxicity, providing the analysis of the mechanism of toxicity data to explore the short-term high-dose medication The safety range; through long-term toxicity in rats, extract understand Curculigo long-term use of high dose and the body to produce toxic side effects of the role of the target organ toxicity, suggesting the drug may produce side effects after stopping a large,The recovery of mouse body; by a martial arts athlete drug test, the subjects observed the physiological function of response, and larger than observed tolerated dose. To determine the toxicity of traditional Chinese medicine Curculigo for clinical applications based on scientific experiments.2,the experimental method2.1 Acute toxicity test method:8-week-old healthy male Wistar rats 40 (200±20g), were randomly divided into control group and high dose group, middle dose group, low dose group,10 in each group. High dose group 1/3h, Interval 5 times; in the dose group 1/5h, Interval3 times; low dose group was given 1. High, medium and low doses of 90g/kg, 54g/kg,18g/kg, clinical adult daily dose is equivalent to 600,360,120 times, continuous observation of 6d, recorded the rats in each group activity, weight and Food intake, visceral organ weighing coefficient determined, organizing to do biopsy to determine the maximum tolerated dose (MTD).2.2 Long-term toxicity experiments in rats:6 weeks old male Wistar rats, were divided into control group and high dose group, low dose group, High and low dose of 15g/kg, 1g/kg of Curculigo water extract, the equivalent of clinical adult dose of 100,58 times daily, a total of 60d, recorded weekly food intake, body weight, blood coagulation time measurement, blood Conventional, chemical and biological indicators, taking visceral organ weighing factor, tissue biopsy done.3,The experimental results3.1 The results of acute toxicity tests3.1.1 behavioral activity of rats is different from the color of which the most obvious shape of stool; last two fed the high dose group, the rats are there different times of abdominal breathing; high, medium dose group Rats 15h, the phenomenon of weak limbs, the next day were back to normal.3.1.2 Rat group body weight, food intake, organ coefficient no significant differences occurred (p> 0.05).3.2 The long-term toxicity experiments in rats3.2.1 High and low dose group among the groups food intake, body weight no significant differences occurred (P> 0.05).3.2.2 the groups was not significant (P> 0.05).3.2.3 routine blood test showed that the measured blood of rats was not significantly different (p> 0.05).3.2.4 Biochemical indicators show that the measured biochemical indicators in each group of aspartate aminotransferase medication control group, high and low dose group had significant difference (p<0.01); recovery in the control group and high dose groups were significantly different (p<0.05), and low dose group were significantly different (p <0.05). ALT recovery, high-dose group and control group, significant differences (p <0.05). Total bilirubin, high-dose group and the control group there were significant differences (p<0.05); other groups no significant differences between (p> 0.05).3.2.5 biopsy revealed, in the high power microscope, cells of various organs in rats and no obvious pathological changes, cell number, shape, size, whether the nuclei consistent with the control group.4,Conclusion4.1 Conclusion of acute toxicityBy toxicity studies confirmed the toxicity of Curculigo extract small clinical dose range of high security, state of the movement taking Curculigo, the effective dose pharmacokinetics and higher.4.2 Long-term toxicity of experimental results in ratsAcute toxicity test, the determination of the indicators no significant differences, the maximum tolerated dose in rats is 90g/kg. Long-term toxicity experiment, rats alanine aminotransferase, aspartate aminotransferase there were significant differences, indicating that long-term use Curculigo extracts, physiological and biochemical function of the liver will cause some adverse reactions.