Prearation And Pharmacodynamics Studyes On Zhizichitang Oral Liquid

Zhizichitang is from the'Shang han lun', and Semen from the composition of the two herbs, has a clear effect of diarrhea mediastinoscopy Yu heat for thermal interference mediastinoscopy, virtual trouble, insomnia, heart upset, irritability and other difficult security Syndrome. Heat treatment of the side is both the basis of party interference mediastinoscopy permits, but also the heat of the treatment based Yang Ming Yu Fang, modern clinic for the treatment of menopausal depression, insomnia, neurasthenia and other diseases, treating diseases as long as consistent with their card machine, but also Modified use of available treatment esophagitis, acute gastritis, cholecystitis and so on. Through extensive literature summary of Zhizichitang is still widely used clinically, and has broad prospects for development and utilization.Zhizichitang has been in clinical use decoction form, according to technical requirements of Chinese drugs, its development into a proprietary oral formulation, while retaining the features and advantages of the original parties on the basis of the taking, storage, carrying more convenient. This paper studies the oral preparation of Zhizichitang, developed a quality control standard, a preliminary stability test, and oral liquid formulations studied animal model of depression, preliminary pharmacodynamic effects.Preparation to the material basis of drug action, multi-index determination, compared the modern technology and traditional techniques to develop a reasonable process route. To genistein, geniposide content indicators, the use of single and multi-factor test method, determine the optimal extraction process:herbs plus 6 times the amount of water, soaking 1.5h, heat reflux extraction 3 times 1 hour; by alcohol precipitation, the extract was purified to determine the water extract was concentrated to the relative density of 1.05-1.15 (80℃), the concentration of 70% ethanol precipitation, standing for 24 hours. Research on manufacturing technology, visit the flavoring agent type and content to determine the prescription, specification, usage and dosage and method of terminal sterilization.10 times the amount prescribed by three batches in a pilot, Zhizichitang proved feasible oral preparation, parameter stability, suitable for mass production.Quality standard, using thin layer chromatography Gardenia Gardenia Black Bean Soup, and oral solution for the qualitative identification of Semen; Determination of total flavonoids; Determination of the content Geniposide. And identification and determination were carried out methodological study. The results showed that:The method is reproducible, specific and strong, precision, recovery rate all meet the requirements. Gardenia Liquid soy soup can effectively control the quality standards for the quality.Preliminary stability studies, three batches of samples at room temperature by 6 months of initial stability test inspection, the quality of the preparation proved stable and controllable.Pharmacodynamic studies of depression model by Zhizichitang oral efficacy in terms of the role of antidepressants. Model of depression induced by reserpine, Zhizichitang oral high, medium and low dose groups could be effective against reserpine-induced hypothermia, high, middle dose group against the ptosis and akinesia; of the desperation caused by acute stress model, Gardenia Liquid soup soy can reduce the immobility time in tail suspension in mice and rats floating in the water fixed time.