Studies On The Preparation Technology And Quallty Standard Of The Effective Partsextracts In Chaiqin Qinggan Formular

Chaiqin Qinggan Formula is composed of Scutellariae, Entrapement, Officinale, Liquorice, and other Chinese medicine, play a role in ShuGan XieYu, qingrejiedu. In the clinical setting Mainly used in the treatment of chronic hepatitis, liver cirrhosis and cholecystitis, and as read the symptoms described below,bitter taste, dry gullet,dazzled, alternate spells of chill and fever, fullness and discomfort in chest and hypochondrium, distending pain in hypochondrium etc. This topic in the traditional Chinese medicine theory as the guiding principles, applying modern pharmaceutical technology, to do the research work of the pre-clinical medicine part to the formula.The main purpose of this paper is to the effective parts of herbs in the formula extraction, refining process, and conducting quality standard research of the effective parts extracts. The main research overview as follows:1. Content determination method research:Establish HPLC, simultaneously determine in ChaiQin qinggan formula Peoni florin, Baicalin and Glycyrrhizin three effective ingredient contents. Use DiamonsilC18 (4.6×250mm,5μm) chromatographic column; Acetonitrile-0.1%phosphoric acid solution as Mobile phase, Gradient elution; detected of dual wavelengths,278nm for Baicalin and Glycyrrhizin,230nm for Paeoniflorin.2. Extraction technology research:Major effective component Baicalin, Glycyrrhizin and Paeoniflorin as index, using the orthogonal experiment, to study water extraction process. Using multiple composition weighted comprehensive mark methods to investigate the best extraction process for: add 8 times medicinal amount of water, heating decocting extract three times, and each extraction 1 hour.3. Refining technology research:Through compare the adsorption and desorption characteristics in the several kinds of macroporous adsorption resin, to Baicalin, Paeoniflorin, Glycyrrhizin and flavonoids in the compound. Separate the effective parts extract optimum resin, and the resin adsorption characteristic and various influencing factors were studied. Optimize of process conditions, get effective parts extracts of best purification refining process conditions. Result:the extracts concentrate to 0.5 g/mL (crude drug) as the sample, in order to 3 mL/min velocity of flow through the AB-8 type of macroporous adsorption resin, with 70% alcohol 5 mL/min velocity elution. Eluent concentrate to the relative density 1.20-1.25 (60℃) thick paste, put into oven, temperature 60℃drying, and get it.4. Effective parts extract quality standards:Directed towards radix bupleuri, radix scutellariae, radix paeoniae alba and radix glycyrrhizae 4 main medicinal materials for TLC identification experiments. Methods operation simple, reproducible and thin-layer chromatogram spots clear. Establish ultraviolet spectrophotometer method, determinate flavonoids,Total iridoid glycosides and total saponin contents, 82.57%of total content. Method of high precision, good reappearance, for ChaiQin qinggan Formula establish the modern quality standards of standardization.