Effects Of Yeast Cell Wall Polysaccharides On Antioxidant Activity And Biosynthesis Of Flavonoids In Chrysanthemum Morifolium

In this study, Chrysanthemum morifolium extracts were tested in vitro antioxidant tests. In order to filter out the best elicitor and the best induction method, we evaluated the effects of different elicitors on antioxidant activity of Chrysanthemum morifolium through this antioxidant model. Fuether work was to evaluate the effects of this elicitor on antioxidant system and biosynthesis of flavonoids in Chrysanthemum morifolium. The main original research results were shown as follows:1. After teated with different elicitors, Leaves of Chrysanthemum morifolium were collected for the determination of total reductive capacity,-OH rdical-scavenging activity and-DPPH radical-scavenging activity respectively. Results indicated that antioxidant activities in leaves of Chrysanthemum morifolium treated with yeast cell wall polysaccharides were significantly increased as compared with those of control. In particular, At the24h after treatment of200mg/L yeast cell wall polysaccharides, total reductive capcity,-OH radical-scavenging activity and-DPPH radical-scavenging activity of the leaves were increased to26.69U/mL,87.12U/mL and77.23%respectively, being about1.91-fold,1.39-fold and1.23-fold of control.2. After yeast cell wall paccharides treatment, total phenols and total flavonoids contents in Chrysanthemum morifolium leaves were significantly increased. It indicated that yeast cell wall polysaccharides had a positive effect on synthesis of phenols and flavonoids in Chrysanthemum morifolium leaves.3. There was a positive correlation (R2=0.9278) between total flavonoid concentration and total antioxidant activity after24h yeast cell wall polysaccharides (500mg/L) treatment. From the results, we speculated that yeast cell wall polysaccharides may increased total antioxidant capacity by increasing concentration of flavonoids in Chrysanthemum morifolium.4. After treatment by yeast cell wall polysaccharides, POD activity in Chrysanthemum morifolium leaves greatly increased, and were always higher than control. POD activity reached the maximum at the48h after treatment. At the24h after treatment, CAT activity in Chrysanthemum morifolium leaves reached the maximum, about1.3-fold of control. Within24h after treatment, PPO activity in Chrysanthemum morifolium leaves was significantly higher than control. The results showed that yeast cell wall polysaccharides coud activate antioxidant enzymes system in Chrysanthemum morifolium leaves.5. Concentrations of chlorogenic acid, luteolin and Linarin in flowers and leaves of Chrysanthemum morifolium were significantly higher than control after treatment by yeast cell wall polysaccharides. It indicated that yeast cell wall polysaccharides had a positive effect on synthesis of chlorogenic acid, luteolin and Linarin in flowers and leaves of Chrysanthemum morifolium.6. Yeast cell wall polysaccharides can significantly improve the key enzyme PAL, C4H activities of flavonoids secondary metabolic pathway in leaves of Chrysanthemum morifolium, indicating that yeast cell wall polysaccharides could activate flavonoids secondary metabolic pathway in leaves of Chrysanthemum morifolium. So, it was presumed that yeast cell wall polysaccharides promoted the accumulation of flavonoids synthesis through activating flavonoids secondary metabolic pathway in leaves of Chrysanthemum morifolium.In summary, yeast cell wall polysaccharides could increase the antioxidant activity of Chrysanthemum morifolium, and had a positive effect on synthesis of the active ingredients of Chrysanthemum morifolium. Therefore, yeast cell wall polysaccharides had a broad prospect to be developed as a new type of green natural elicitor used in secondary metabolites regulation of medicinal plants.