Effective Component Separation And Quality Evaluation Of Huangshan Tribute Chrysanthemum (Chrysanthemum Morifolium Ramat.)

As one of bulk common medical herbs with profoundly medical history, Chrysanthemum which belongs to flower head of compositae was widely applied to various fields. The market demand of Chrysanthemum is increasing on account of the function of heat-clearing, detoxifying, calming liver, brighten eyes and so on. The stability of medical quality goods was easily influenced by great difference of chrysanthemum medical material components and the distinction of chrysanthemum varieties and origins. At present the study of chrysanthemum focuses on several components (such as chlorogenic acid, Luteoloside).To have comprehensively control qualitystandards of HuangShan GJ,13HuangShan GJ samples were collected from four main lands (HuangShan three county area).First, several single compounds were separated and purified from Huangshan GJ, and then the fingerprint study were followed.The above process were aimed at identifying the quality characteristics of HuangShan GJ.First, using70%alcohol solution ultrasonic extraction of this topic. Eight monomercompounds were separated and purified by column chromatography with macroporousresin, RP-C18and Sephadex LH-20of such modern chromatographic separationtechnology from HuangShan GJ, and studying phenolic acids and flavonoids of chemicalactive ingredients. The structures of the obtained compounds were elucidated on the basisof spectroscopic methods, including UV、1H-NMR、13C-NMR、MS and HRMS, togetherwith chromatographic methods, such as TLC and HPLC. They were chlorogenic acid (1),4,5-di-O-caffeoylquinic acid (2), luteolin-7-O-6″-malonylglucoside (3), luteolin7-O-β-D-glucoside (4),3,5-di-O-caffeoylquinic acid (5),3,4,5-tri-O-caffeoylquinic acid(6),1,5-di-O-caffeoylquinic acid (7) and3,4-di-O-caffeoylquinic acid (8), ConclusionsCompound3was isolated from Huangshan GJ for the first time.13fingerprints of HuangShan GJ were established through the method of HPLC(highperformance liquid chromatography). The HPLC conditions were as follows: ZorbaxEclipse Plus Phenyl-Hexyl as separation column,0.1%formic acid aqueous solution-0.1%formic acid acetonitrile system as mobile phasewith gradient elution, flowing at therate of1.0mL/min,327nm as the optimum detection wavelength,30℃as the optimumColumn temperature,5μL as injection quantity. The similarity of Chromatographic samples was calculated by―Similarity Evaluation System for Chromatographic Fingerprintof Traditional Chinese Medicine (Version2004A)‖written by Pharmacopeia committee,and the results of the similarity showed: the similarity of the chromatographic fingerprintsof the13samples was between0.96and1.00, while the level of similarity with samples ofthe other sorts was below0.86. Fingerprints of GJ were characterized with accuracy,stability and reproducibility, and it provides the criterion for quality control anddiscrimination of Chrysanthemum.