Compositon And Antioxidant Activity Of Ganoderma Lucidum Polysaccharides Produced In Liquid Culture With Oats

Ganoderma lucidum polysaccharide is a main effective component of Ganodermalucidum, which has many kinds of biological activities such as antioxidation, immuneregulation, antitumor, reduce blood sugar, reduce blood fat, et al. Artificial cultivation ofGanoderma lucidum is costly and complex, the production cycle of which is long and theprocess of which is hard to control. Liquid culture is a good method to produce Ganodermalucidum base on its advantages of lower cost, simpler technology, shorter production cycleand controllable process. At present, synthetic medium and semisynthetic medium are themain medium of liquid culture for Ganoderma lucidum, while grains are rarely used as themedium. The thesis studied the composition and antioxidant activity of Ganoderma lucidumpolysaccharides produced both in liquid culture with oats and solid culture with cottonseedhulls. Antioxidant activity of polysaccharides was evaluated by means of DPPH,·OH andreducing power. The primary results were as followed:(1) The cell biomass of Ganoderma lucidum achieve the maximum after cultivated for3-4days in oat culture, or7days in semisynthetic culture, while the maturity of fruit body ofGanoderma lucidum needs90-110days.(2) The average purity of water soluble polysaccharides produced from oat culture was58.79%, which was2.1times higher than those produced from solid culture and0.1timeslower than those produced from semisynthetic culture. The average purity of alkaline solublepolysaccharides produced from oat culture was25.08%, which was0.67times lower thanthose produced from solid culture and0.11times lower than those produce fromsemisynthetic culture. The average purity of extracellular polysaccharides was81.10%, whichwas1.01times higher than those produced from semisynthetic culture. In the sameconcentration, the average antioxidant activity of water soluble polysaccharides producedfrom oat culture was lower than those produced from solid culture, while the averageantioxidant activity of alkaline soluble polysaccharides produced from oat culture was higherthan those produced from solid culture. Both the water soluble polysaccharides and thealkaline soluble polysaccharides produced from oat culture had a higher antioxidant activitythan those produced from semisynthetic culture. Among the three kinds of oat culture, thegerminant oat culture could produce the highest antioxidant polysaccharides, secondly the zymolytic oat, finally the gelatinized oat.(3) The polysaccharides were seperated using DEAE-cellulose. Polysaccharidesextracted from fruit body had4compositions. Water soluble polysaccharides and alkalinesoluble polysaccharides produced from germinant oat culture had3compositions. Otherpolysaccharides had2compositions. Of all the compositions, the0.25mol/L NaCl elutionfraction showed the highest antioxidant activity, Next were the0.1mol/L NaCl elution fractionand the0.5mol/L NaCl elution fraction, finally the water elution fraction.