Ganoderma Lucidum In-depth Liquid Fermentation Process And Lanthanon Influence Research

The Ganoderma lucidum is one of famous fungi for medicinal purpose in our country since the ancient times. The G. lucidum contains many kinds of physiological regulation factors which have the obvious function effect to the human body. G. lucidum polysaccharide is G. lucidum's main active material which is the material base of G. lucidum giveing to play its effect.The technology of liquid in-depth fermentation belongs to one of modern biological technologies.In the last few years, there are more and more the research of G. lucidum by liquid in-depth fermentation. The people have done much beneficial study on the G. lucidum's culture medium and the fermentation condition. But there are different opinions about how to determine the best culture medium as well as to optimize the fermentation condition. At the same time,Lanthanon has been demonstrated its superiority in increasing the production, high quality, resistance and enhancing the economic efficiency when they are used in the edible fungus, which has the important significance to improve the growth condition of the edible fungus, and enhance its nutritional value. But the research work of the edible fungus is not sufficient and thorough, and there are few varieties of the edible fungus and Lanthanon in the research work. Using superiority of the rare earth is not yet developing completely. The related application and the basic research work need to continues to enhance. In order to exert the superiority of Lanthanon resources in Ganzhou.This experiment has done the careful research on the optimization of the culture medium and fermentation condition and influence rule of Lanthanon.The effect of nutrient demands and environment conditions on the mycelial biomass and extracellular polysaccharide were studied respectively by single factor experiments and orthogonal test. The experimental results showed that the optimum formula of liquid culture medium is soluble starch 5%, soybean cake powder 5%, KH2PO40.10%, MgSO40.09%, and the fermentation conditions is as follow: initial pH 5.5~6.0,medium capacity 32%(80mL/250mL flask), rev of 150rpm, no dispersing agent, culture time 8d.Under optimizing culture medium and condition, this experiment plans to culture G. lucidum hypha by joining the different densities of Lanthanum Nitrate, Cerium Nitrate, Antiscorbutic Acid Lanthanum and Antiscorbutic Acid Cerium, in order to seek the best raise condition. This findings indicated:In liquid culture process, different density of Lanthanon have different effects on the mycelial biomass and the plysaccharide in its spore and and the total polysaccharide which have certain degree promoter among 50~300mg/L. The mycelial biomass enhances separately:36.03%, 55.24%, 64.48%, 76.58%, the polysaccharide in the G. lucidum spore is: 31.48%, 42.27%, 49.98%, 65.52%, and the total polysaccharide of G. lucidum is 24.73%,32.26%,30.95%,30.78%. But the effects of different density of the rare-earth elements on the extracellular polysaccharide are not very obvious.The experiment indicated suitable density of Lanthanon can enhance the G. lucidum the biomass and the quality(the plysaccharide).It is beneficial element of G. lucidum.